| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,399,985 | (C)7→8 | coding (252/516 nt) | ogt ← | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,399,978 | 1 | . | C | 97.0% | 110.1 / ‑3.1 | 33 | coding (259/516 nt) | ogt | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (16/16); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 | |||||||||||
ATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC > NC_000913/1399844‑1400111 | aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTAAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑cccc > 1:299700/1‑139 (MQ=255) ccGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccccccccgta < 1:304151/139‑5 (MQ=255) tGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGt < 1:180669/139‑1 (MQ=255) cGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTggg < 1:306660/139‑1 (MQ=255) gCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGa < 2:784236/139‑1 (MQ=255) aCCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAg < 1:367991/139‑1 (MQ=255) cAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCg < 1:468875/139‑1 (MQ=255) ttGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCtt > 1:4547/1‑139 (MQ=255) cATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCg > 1:542838/1‑139 (MQ=255) ccTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGt < 2:162005/71‑1 (MQ=255) ccTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGt > 1:162005/1‑71 (MQ=255) ccTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTg > 2:701107/1‑139 (MQ=255) ccTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTg > 2:291071/1‑139 (MQ=255) cccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGAtt < 2:4547/139‑1 (MQ=255) ccGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATtg > 2:776010/1‑139 (MQ=255) cGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccccccccg > 2:292926/1‑56 (MQ=255) cGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccccccccg < 1:510986/58‑3 (MQ=255) cGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑ccccccccg < 1:292926/58‑3 (MQ=255) cGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCACTGAAATGGCCT‑ccccccccg > 2:510986/1‑56 (MQ=255) aGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTggg < 1:393962/58‑1 (MQ=255) aGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTggg > 2:393962/1‑58 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCaa < 2:285048/88‑1 (MQ=255) tGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCaa > 1:285048/1‑88 (MQ=255) gCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATTCTAAGATTACCGGCAAAATATCCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgcg > 1:19119/1‑139 (MQ=255) gCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATtggtgg > 2:164865/1‑129 (MQ=255) gCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATtggtgg < 1:164865/129‑1 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAg < 1:48176/54‑1 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAg > 2:48176/1‑54 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgc > 1:50829/1‑134 (MQ=255) aGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATgc < 2:50829/134‑1 (MQ=255) tgtTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATa > 2:55164/1‑139 (MQ=255) tCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAg > 1:130482/1‑47 (MQ=255) tCCAGACTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAg < 2:130482/47‑1 (MQ=255) aCTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATg < 2:79313/121‑1 (MQ=255) aCTTCGCGCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATg > 1:79313/1‑121 (MQ=255) cgcgCTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGAt > 1:770747/1‑139 (MQ=255) gcgcTGAAATGGCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATa > 2:591276/1‑139 (MQ=255) ggCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGTGCTCATAGCCTTCTTTGCGATAATGGATGTcc < 1:291071/139‑1 (MQ=255) ggCGTCCCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTcc < 1:701107/139‑1 (MQ=255) | ATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGT‑CCCCCCCGTAGCAGTGGGAAGCGTATCAATAATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCC > NC_000913/1399844‑1400111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |