Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,700,201 | (T)5→6 | coding (847/1593 nt) | malX → | maltose and glucose‑specific PTS enzyme IIB component and IIC component |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,700,196 | 1 | . | T | 100.0% | 65.3 / NA | 22 | G281V (GGT→GTT) | malX | maltose and glucose‑specific PTS enzyme IIB component and IIC component |
Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (9/13); total (9/13) |
TCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑G‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCT > NC_000913/1700074‑1700328 | tCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAgcgc < 1:119022/139‑1 (MQ=255) gtggCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTc > 2:140927/1‑139 (MQ=255) gCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCtt > 1:487132/1‑139 (MQ=255) cATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCttt > 2:795965/1‑139 (MQ=255) aTTCGCTTTACCGACGCGGGCGGCACGCAGGAAGTCTACGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCaa < 1:795965/139‑1 (MQ=255) cGACCCAGGGGGCAAGCAGGGAGTCTGCGGTCAAACCGTCAGCGGTGCACGCACCATCCACCAGGCGCAATTTACTTGGGCTACCACTCACGTT‑TTTTTCTTAAAGCGCCGTGCGTTTCCTCT‑GCCAGGGAAAAtactc > 1:258839/1‑135 (MQ=255) cGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTcggcg < 2:487132/139‑1 (MQ=255) cAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGt < 1:140927/139‑1 (MQ=255) gTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCa > 1:64966/1‑139 (MQ=255) gCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTg < 1:307511/139‑1 (MQ=255) ccGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg < 2:613902/139‑1 (MQ=255) ccGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg < 2:90971/139‑1 (MQ=255) gcgcACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg < 1:62598/130‑1 (MQ=255) gcgcACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAg > 2:62598/1‑130 (MQ=255) ccAGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGa > 2:504119/1‑139 (MQ=255) aGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGt < 1:336015/109‑1 (MQ=255) aGGCGCAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGACTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGt > 2:336015/1‑109 (MQ=255) cAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGt < 1:711836/104‑1 (MQ=255) cAATTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGt > 2:711836/1‑104 (MQ=255) aaTTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCt < 1:676718/108‑1 (MQ=255) aaTTGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCt > 2:676718/1‑108 (MQ=255) ttGAGTTGCCCGACCACTCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCAt < 2:64966/139‑1 (MQ=255) tCACG‑GTTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTctgct < 2:797849/139‑1 (MQ=255) | TCACATTCTGGTGGCATTAATTCGCTTTACCGACGCAGGCGGCACGCAGGAAGTCTGCGGTCAAACCGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACG‑G‑TTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCT > NC_000913/1700074‑1700328 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |