| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,950,559 | (C)5→6 | intergenic (‑37/‑273) | aspS ← / → yecD | aspartyl‑tRNA synthetase/isochorismatase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,950,554 | 1 | . | C | 96.5% | 98.9 / ‑3.1 | 29 | intergenic (‑32/‑278) | aspS/yecD | aspartyl‑tRNA synthetase/isochorismatase family protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (15/13); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
GAAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑CCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAC > NC_000913/1950418‑1950683 | gAAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑cc > 1:758629/1‑139 (MQ=255) cAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑cccccctg < 1:679134/139‑4 (MQ=255) tCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGAc > 1:486615/1‑139 (MQ=255) cACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGAcc < 1:209131/139‑1 (MQ=255) gacgacgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAg > 2:172103/1‑139 (MQ=255) cgGTTGACCCAACCACACCGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTCTACGCAAGAGCTATACCTTAACTTAGCTGTCGGCGGATGACCCCC‑TGCTCCGCAGCTGACTAAGTAGCa > 1:340782/1‑139 (MQ=255) gTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCGGc < 1:439961/139‑1 (MQ=255) gACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGtt < 1:172103/139‑1 (MQ=255) cccAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAg < 2:486615/139‑1 (MQ=255) aaCCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg < 1:234911/139‑1 (MQ=255) cACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTa > 1:164796/1‑128 (MQ=255) cACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTa < 2:164796/128‑1 (MQ=255) cTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc > 2:22381/1‑122 (MQ=255) cTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 1:22381/122‑1 (MQ=255) tGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTa < 2:758629/139‑1 (MQ=255) aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCt > 2:646574/1‑139 (MQ=255) aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCt > 2:301671/1‑139 (MQ=255) aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCt > 1:93874/1‑139 (MQ=255) atCCCTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCcgc > 1:405748/1‑139 (MQ=255) ccTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAg < 2:322691/61‑1 (MQ=255) ccTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAg > 1:322691/1‑61 (MQ=255) ccTTAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCt > 2:404469/1‑139 (MQ=255) ttAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTgg < 2:572897/139‑1 (MQ=255) tAACTTAGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGa > 2:639747/1‑139 (MQ=255) aGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCt > 1:786804/1‑139 (MQ=255) aGCTGCCGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCt > 1:288128/1‑139 (MQ=255) ccGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa > 1:492236/1‑139 (MQ=255) ccGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa < 2:405748/139‑1 (MQ=255) ccGGCGGATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa < 2:786804/139‑1 (MQ=255) cggcggATG‑CCCCCGTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGTATGAAAAAAGGCGGCTATTATACTTGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAc < 2:492236/139‑1 (MQ=255) ggcggATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa > 1:512776/1‑137 (MQ=255) ggcggATGCCCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa < 2:512776/137‑1 (MQ=255) | GAAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑CCCCC‑TGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAC > NC_000913/1950418‑1950683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |