| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,767,445 | (C)7→8 | intergenic (+90/‑265) | rnlB → / → yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,767,438 | 1 | . | C | 86.1% | 127.1 / 12.5 | 43 | intergenic (+83/‑272) | rnlB/yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
| Reads supporting (aligned to +/- strand): ref base . (6/0); new base C (16/21); total (22/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAA > NC_000913/2767304‑2767567 | ctctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑c‑cc‑c < 2:722674/139‑1 (MQ=255) tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑c‑cc‑cc > 2:442012/1‑139 (MQ=255) tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑c‑cc‑cc > 1:801038/1‑139 (MQ=255) cccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGt > 2:467384/1‑139 (MQ=255) ccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTc < 1:562807/139‑1 (MQ=255) ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAac < 2:801038/139‑1 (MQ=255) gTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAA‑‑A‑CA‑TCAC‑TCACCAAGTCCTGCATAGGTAACACGt > 2:314256/1‑139 (MQ=255) tGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTc > 2:113401/1‑139 (MQ=255) tCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTccc < 2:500254/139‑1 (MQ=255) cAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTcccc < 1:113401/139‑1 (MQ=255) ggATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTccc > 1:159428/1‑134 (MQ=255) ggATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTccc < 2:159428/134‑1 (MQ=255) ggATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCtgtg > 2:685733/1‑139 (MQ=255) ttttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc < 1:442012/139‑1 (MQ=255) ttttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc < 1:467384/139‑1 (MQ=255) tttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAccc < 1:685733/139‑1 (MQ=255) tctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTccc < 1:12975/139‑1 (MQ=255) tctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTccc < 1:558137/139‑1 (MQ=255) ccAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑C‑CCACACC‑ACACAAATTCCCCCATAGCGAACAAGTGTTCCCCTGTGCACCCACTACTAAATTCCCTCCCAATTaaa > 1:53571/1‑139 (MQ=255) ttCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑C‑CC‑CCCCACCATGACGTCCTGCCTATGGAACACGTTTTCCCTTGTTGTCACACAACTAAAATTCCTCCCCTTTAACATTCGCaataa > 2:714778/1‑139 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAA‑‑C‑CC‑CCCC‑TCACAACGCACTGCTTAGGGAACAAGTTTACCTATGTGAACCCACGACTAAATTTCGCCCCCTTCATACAACTAAATACTGt > 2:171808/1‑139 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAac > 1:113040/1‑80 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAac < 2:113040/80‑1 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑CACC‑CCCC‑ACACAACGTCATGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTCAATTTCCCCCCCTTTAAACTTCTCAATAATga > 1:271537/1‑138 (MQ=255) ttttGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGACCTGCATAGGGAACACGTCTTCCCCTGTGCACCCACGAATACCTTTCCCCCCCTTTAACCTGCTCAATAATGTCAcc < 1:314256/139‑1 (MQ=255) gATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACCACGTCCTGCATAGGGCACACGTTTTCCCCTGTGCACCCACGCCTAAATTTCCCCCCCTTTAAACTTATCAACAATGTCACCTATTTTCTg > 2:400370/1‑139 (MQ=255) tGTCCAATATGATCAAATAGCATT‑AA‑‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCTTGTGCACCGGCGACTAAATTTCCCCCCCTTTACACTTCTCAATAATCTCACCAATTTTCTGGAAATCCTacg > 2:540089/1‑137 (MQ=255) tCCAATATGATCAAATAGCATT‑AACCC‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg < 2:447612/129‑1 (MQ=255) tCCAATATGATCAAATAGCATT‑AACCC‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAAAACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg > 1:447612/1‑129 (MQ=255) tCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTc < 2:527722/42‑1 (MQ=255) tCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTc > 1:527722/1‑42 (MQ=255) tCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatga > 1:635122/1‑139 (MQ=255) tCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTat < 2:130633/137‑1 (MQ=255) tCCAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCACTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTat > 1:130633/1‑137 (MQ=255) cAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgatg > 1:233098/1‑139 (MQ=255) cAATATGATCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgatg < 1:171808/139‑1 (MQ=255) aTCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTcc < 1:694141/139‑1 (MQ=255) tCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGtttt < 1:284861/52‑1 (MQ=255) tCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTccc < 1:530908/55‑1 (MQ=255) tCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTccc > 2:530908/1‑55 (MQ=255) tCAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACCTCCTGCATAGGGAACACGtttt > 2:284861/1‑52 (MQ=255) cAAATAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCg < 2:53571/139‑1 (MQ=255) aaTAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTc > 2:275956/1‑50 (MQ=255) aaTAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTc < 1:275956/50‑1 (MQ=255) tAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGTACCCACGACTAACTTTCCCCCCCTTTAAACTTCTCAATAATTTAACCAATTTTTTGGAAATCCTTTAATGCCTTTCCCGGTaa > 1:697862/1‑139 (MQ=255) tAGCATT‑AAC‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGATTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTaa > 2:414482/1‑139 (MQ=255) | CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑C‑CC‑CCCC‑TCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAA > NC_000913/2767304‑2767567 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |