Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,981,901	(C)5→6	coding (282/1419 nt)	araE ←	arabinose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,981,896	1	.	C	100.0%	180.4 / NA	50	A96G (GCC→GGC)	araE	arabinose transporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (26/24);  total (26/24)

TGCCATTTCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGG‑CCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCAC  >  NC_000913/2981760‑2982020                                                                                                                                          |                                                                                                                             tGCCATTTCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGG‑cc                                                                                                                            >  2:256377/1‑139 (MQ=255)      tttCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGG‑ccccccg                                                                                                                       <  2:150425/139‑3 (MQ=255)         cAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGG‑ccccccgcca                                                                                                                    <  1:203353/139‑6 (MQ=255)          aGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCAt                                                                                                                    <  1:227600/139‑1 (MQ=255)          aGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCAt                                                                                                                    <  1:553580/139‑1 (MQ=255)              aGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAgg                                                                                                                <  1:256377/139‑1 (MQ=255)                aTACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCt                                                                                                              <  1:56849/139‑1 (MQ=255)                         agCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACg                                                                                                     >  1:539131/1‑139 (MQ=255)                          gCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGc                                                                                                    >  1:790873/1‑139 (MQ=255)                           cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGcc                                                                                                   <  2:539131/139‑1 (MQ=255)                           cGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGcc                                                                                                   >  1:385482/1‑139 (MQ=255)                                        gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCAt                                                                                                                    <  2:520011/109‑1 (MQ=255)                                        gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCAt                                                                                                                    >  1:520011/1‑109 (MQ=255)                                        gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGa                                                                                      >  2:421700/1‑139 (MQ=255)                                        gATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGa                                                                                      >  1:257508/1‑139 (MQ=255)                                          tCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACa                                                                                    >  2:153405/1‑139 (MQ=255)                                             cGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                 <  2:5574/139‑1 (MQ=255)                                                    aaTGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCAtt                                                                          <  1:421700/139‑1 (MQ=255)                                                      tGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTaa                                                                        >  1:30611/1‑139 (MQ=255)                                                                cacaCGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCAcc                                                              <  2:257508/139‑1 (MQ=255)                                                                cacaCGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCAcc                                                              <  1:514853/139‑1 (MQ=255)                                                                  cacGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCaa                                                            >  1:158531/1‑139 (MQ=255)                                                                  cacGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCaa                                                            >  2:554556/1‑139 (MQ=255)                                                                       gcggcgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgct                                                       <  1:504442/139‑1 (MQ=255)                                                                       gcggcgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATtgct                                                       >  1:610886/1‑139 (MQ=255)                                                                          gcgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCa                                                    >  1:381936/1‑139 (MQ=255)                                                                          gcgATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCa                                                    <  2:30611/139‑1 (MQ=255)                                                                                  cATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGcat                                            <  2:790873/139‑1 (MQ=255)                                                                                    tctcTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGcatca                                          <  1:257103/139‑1 (MQ=255)                                                                                      tctACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATg                                        <  1:197993/139‑1 (MQ=255)                                                                                               gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                 <  2:330630/89‑1 (MQ=255)                                                                                               gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                 >  1:330630/1‑89 (MQ=255)                                                                                               gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                 <  1:496289/89‑1 (MQ=255)                                                                                               gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGcc                                                                                 >  2:496289/1‑89 (MQ=255)                                                                                               gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCaa                                                                               >  1:733475/1‑91 (MQ=255)                                                                                               gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCaa                                                                               <  2:733475/91‑1 (MQ=255)                                                                                               gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGGGCATCATGCTACTAacc                               >  2:666505/1‑139 (MQ=255)                                                                                               gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAg                                               >  1:644173/1‑123 (MQ=255)                                                                                               gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAg                                               <  2:644173/123‑1 (MQ=255)                                                                                               gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAacc                               >  1:161022/1‑139 (MQ=255)                                                                                               gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAacc                               >  1:529865/1‑139 (MQ=255)                                                                                               gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGG‑ccccccg                                                                                                                       >  1:565917/1‑48 (MQ=255)                                                                                               gTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGG‑ccccccg                                                                                                                       <  2:565917/50‑3 (MQ=255)                                                                                                tCGCAAAAGCGGACCATATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAacca                              >  1:537668/1‑139 (MQ=255)                                                                                                  gCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAaccacc                            <  1:554556/139‑1 (MQ=255)                                                                                                    aaaaGCGGACCCTATAGAACCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCa                          >  2:193038/1‑139 (MQ=255)                                                                                                                     aaCCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTgg         <  1:56505/139‑1 (MQ=255)                                                                                                                      aCCGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGt        <  2:610886/139‑1 (MQ=255)                                                                                                                       ccGAGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTc       <  1:193038/139‑1 (MQ=255)                                                                                                                          aGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGc    >  2:200307/1‑139 (MQ=255)                                                                                                                          aGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGc    >  2:584558/1‑139 (MQ=255)                                                                                                                          aGTACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGc    >  2:634380/1‑139 (MQ=255)                                                                                                                            tACAAACAGGATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGcac  >  2:479708/1‑139 (MQ=255)                                                                                                                                     gATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAgcg                                                                  >  1:88198/1‑66 (MQ=255)                                                                                                                                     gATGGCCCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAgcg                                                                  <  2:88198/66‑1 (MQ=255)                                                                                                                                          |                                                                                                                             TGCCATTTCAGAAAGATACAGAGGAGCGGTGTAAGACGCGATCCCGACAGCAATGCCCAGCACCACACGAGCGGCGATTAACATCTCTACGCTGGTCGCAAAAGCGGACCCTATAGAACCGAGTACAAACAGGATGG‑CCCCCGCCATCAGGCTGTATTTACGCCCCAGGCGGAACGACAGCCAACCATTAAACAGCGCACCAATTGCTGCACCGAGCATCATGCTACTAACCACCCATTCCTGCAAACGACTGGTCAGCAC  >  NC_000913/2981760‑2982020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑