Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,984	(G)7→8	coding (1041/1245 nt)	sstT →	sodium:serine/threonine symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,977	1	.	G	100.0%	185.0 / NA	51	A345G (GCG→GGG)	sstT	sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (22/29);  total (22/29)

GGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTC  >  NC_000913/3240843‑3241109                                                                                                                                        |                                                                                                                                     ggcgggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑gggg                                                                                                                                  <  2:367216/139‑1 (MQ=255) ggcgggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑gggg                                                                                                                                  <  2:208269/139‑1 (MQ=255)  gcgggccCAGCAATCACTATTTCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGGGGTGGCTTCTCTGTGTGCCTGTGGCCGATCCGG‑GCGgg‑gggggg                                                                                                                                >  1:244226/1‑139 (MQ=255)    gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggg                                                                                                                               <  1:63848/139‑1 (MQ=255)     ggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCCCGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑gggggggg                                                                                                                              >  1:682155/1‑138 (MQ=255)      gcgcAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggg                                                                                                                             >  2:80201/1‑137 (MQ=255)      gcgcAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑gggg                                                                                                                                  <  2:18647/134‑1 (MQ=255)      gcgcAGCAATCACCATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCAGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑gggg                                                                                                                                  >  1:18647/1‑134 (MQ=255)           gcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCTACGGCGATGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctg                                                                                                                         <  1:290405/139‑1 (MQ=255)                cACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctgctgct                                                                                                                    <  1:219228/139‑1 (MQ=255)                       aCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCa                                                                                                             <  1:778581/139‑1 (MQ=255)                       aCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCa                                                                                                             <  2:682155/139‑1 (MQ=255)                       aCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCa                                                                                                             <  2:261903/139‑1 (MQ=255)                         cGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACt                                                                                                           <  2:428635/139‑1 (MQ=255)                          gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTg                                                                                                          >  1:543735/1‑139 (MQ=255)                          gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTg                                                                                                          >  2:292132/1‑139 (MQ=255)                          gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTg                                                                                                          <  1:746856/139‑1 (MQ=255)                           tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctg                                                                                                                         >  2:223848/1‑123 (MQ=255)                           tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctg                                                                                                                         <  1:223848/123‑1 (MQ=255)                           tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTgg                                                                                                         >  1:6623/1‑139 (MQ=255)                           tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTgg                                                                                                         >  1:689033/1‑139 (MQ=255)                           tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTgg                                                                                                         >  2:209799/1‑139 (MQ=255)                           tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTgg                                                                                                         >  2:109900/1‑139 (MQ=255)                           tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTgg                                                                                                         >  1:774102/1‑139 (MQ=255)                            gtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                        <  1:80201/139‑1 (MQ=255)                            gtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                        >  1:446697/1‑139 (MQ=255)                             ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                        <  2:715641/138‑1 (MQ=255)                             ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                        >  1:715641/1‑138 (MQ=255)                                          cGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCg                                                                                          <  2:543735/139‑1 (MQ=255)                                                 tACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTc                                                                                   <  1:75805/139‑1 (MQ=255)                                                  aCGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCg                                                                                  <  1:209799/139‑1 (MQ=255)                                                      tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTg                                                                                                          >  2:525624/1‑111 (MQ=255)                                                      tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTg                                                                                                          <  1:525624/111‑1 (MQ=255)                                                         gTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGata                                                                           >  1:778375/1‑139 (MQ=255)                                                          tATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGatat                                                                          <  2:774102/139‑1 (MQ=255)                                                                  tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGcc                                                                                                       <  1:376874/102‑1 (MQ=255)                                                                  tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGcc                                                                                                       >  2:376874/1‑102 (MQ=255)                                                                        tGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtgg                                                            <  2:244226/139‑1 (MQ=255)                                                                              cGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                             >  1:489818/1‑116 (MQ=255)                                                                              cGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                             <  2:489818/116‑1 (MQ=255)                                                                               ggCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGt                                                     <  2:259143/139‑1 (MQ=255)                                                                                gcgcTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTc                                                    <  1:753116/139‑1 (MQ=255)                                                                                    tgctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCt                                                >  2:799512/1‑139 (MQ=255)                                                                                             gCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCg                                       <  1:799512/139‑1 (MQ=255)                                                                                              cGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCgg                                      >  2:574882/1‑139 (MQ=255)                                                                                              cGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑gggg                                                                                                                                  <  1:558165/46‑1 (MQ=255)                                                                                              cGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑gggg                                                                                                                                  >  2:558165/1‑46 (MQ=255)                                                                                                tggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                                    <  2:446697/139‑1 (MQ=255)                                                                                                 ggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCTCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGt                                   <  1:574882/139‑1 (MQ=255)                                                                                                   tggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTAt                                 >  1:712327/1‑139 (MQ=255)                                                                                                           ctGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGAc                         >  2:397208/1‑139 (MQ=255)                                                                                                               gtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAat                                                                                                 <  1:587250/63‑1 (MQ=255)                                                                                                               gtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAat                                                                                                 >  2:587250/1‑63 (MQ=255)                                                                                                               gtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTAATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCtt                     >  1:567737/1‑139 (MQ=255)                                                                                                                      tgGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCa                                                                                                             >  2:331088/1‑44 (MQ=255)                                                                                                                      tgGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCa                                                                                                             <  1:331088/44‑1 (MQ=255)                                                                                                                          gcATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACcgcg          <  1:292132/139‑1 (MQ=255)                                                                                                                          gcATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACcgcg          >  1:493348/1‑139 (MQ=255)                                                                                                                                ggCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAAc    <  2:778375/139‑1 (MQ=255)                                                                                                                                  cGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAActc  <  2:712327/139‑1 (MQ=255)                                                                                                                                  cGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAActc  <  1:397208/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     GGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTC  >  NC_000913/3240843‑3241109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑