| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,432,994 | (C)6→7 | coding (567/1125 nt) | smf ← | DNA recombination‑mediator A family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,432,988 | 1 | . | C | 97.4% | 129.8 / ‑3.3 | 38 | coding (573/1125 nt) | smf | DNA recombination‑mediator A family protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (20/17); total (20/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑G‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTACACCATCG > NC_000913/3432850‑3433117 | tGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgc‑g > 1:13421/1‑139 (MQ=255) tCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑G‑ccccc < 1:119217/139‑1 (MQ=255) aaGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGACAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAg < 1:250501/139‑1 (MQ=255) aGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGACAGc > 2:38461/1‑139 (MQ=255) caccaccaGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGc < 1:536310/139‑1 (MQ=255) caGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcg > 2:66318/1‑139 (MQ=255) aGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcgg < 1:597709/139‑1 (MQ=255) tACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcggcg < 1:66318/139‑1 (MQ=255) tACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAcggcg > 2:209215/1‑139 (MQ=255) cTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGAt > 2:380437/1‑139 (MQ=255) ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCAC‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGAAGGCgggtagga < 1:466043/139‑6 (MQ=255) ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGa > 2:30821/1‑139 (MQ=255) ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGa > 1:677363/1‑139 (MQ=255) tACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGaa > 2:458712/1‑139 (MQ=255) aCTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAAt < 2:677363/139‑1 (MQ=255) tAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGt > 2:146237/1‑139 (MQ=255) aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCa > 2:77338/1‑96 (MQ=255) aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCa < 1:77338/96‑1 (MQ=255) cTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAA‑GGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc > 1:428291/1‑139 (MQ=255) gATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTcc > 2:554728/1‑139 (MQ=255) aTAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa < 1:30821/139‑1 (MQ=255) gCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc < 2:353877/139‑1 (MQ=255) cGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑G‑cccccc > 1:611171/1‑63 (MQ=255) cGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑G‑cccccc < 2:611171/63‑1 (MQ=255) aaTTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGa < 2:324499/139‑1 (MQ=255) aTTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGAc > 2:199211/1‑139 (MQ=255) ttGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACg < 2:13421/139‑1 (MQ=255) tGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGc < 1:205287/139‑1 (MQ=255) ggggTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCt > 2:308255/1‑139 (MQ=255) gggTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTAGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTg > 1:551958/1‑139 (MQ=255) gTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTa < 1:380437/139‑1 (MQ=255) gTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATCGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTa > 1:124983/1‑139 (MQ=255) gAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAgg < 2:390091/139‑1 (MQ=255) gAACATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAgg > 1:548206/1‑139 (MQ=255) aaCATCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGgc < 1:209215/139‑1 (MQ=255) aTCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc < 2:761283/93‑1 (MQ=255) aTCGAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc > 1:761283/1‑93 (MQ=255) gAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCAt < 1:691417/63‑1 (MQ=255) gAGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCAt > 2:691417/1‑63 (MQ=255) aGGGGAAATTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTa > 2:220372/1‑139 (MQ=255) aaTTCCGAGACGAGAGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCt < 2:548206/139‑1 (MQ=255) acgagaGC‑GCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTACACCATCg > 2:226095/1‑139 (MQ=255) | TGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGC‑G‑CCCCCCTGTTCAAGCAGACTGGCAGCCAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTACACCATCG > NC_000913/3432850‑3433117 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |