| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,897,050 | +G | coding (277/666 nt) | yieH → | phosphoenolpyruvate and 6‑phosphogluconate phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,897,046 | 1 | . | G | 96.4% | 193.6 / ‑0.4 | 56 | coding (273/666 nt) | yieH | phosphoenolpyruvate and 6‑phosphogluconate phosphatase |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (23/31); total (24/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑GGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCT > NC_000913/3896908‑3897179 | tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa < 2:727245/139‑1 (MQ=255) ggTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑gggggct > 1:6261/1‑136 (MQ=255) aaaCTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAgcg < 2:727227/139‑1 (MQ=255) aCTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCt < 1:43346/139‑1 (MQ=255) cTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTc < 2:37439/139‑1 (MQ=255) cTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTc < 2:656954/139‑1 (MQ=255) gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCt > 1:532156/1‑139 (MQ=255) gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTCCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCt > 1:574885/1‑139 (MQ=255) tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa > 1:694498/1‑120 (MQ=255) tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa < 2:225177/120‑1 (MQ=255) tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa > 1:225177/1‑120 (MQ=255) tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa < 2:694498/120‑1 (MQ=255) aCGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGt < 1:70881/139‑1 (MQ=255) tATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGAt < 2:459344/139‑1 (MQ=255) tCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtg < 1:130001/139‑1 (MQ=255) cccTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgt < 2:177282/139‑1 (MQ=255) cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgt > 2:368673/1‑139 (MQ=255) ttGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg < 2:368856/139‑1 (MQ=255) ttGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg < 2:157282/139‑1 (MQ=255) ttGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg > 2:235238/1‑139 (MQ=255) ttGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg < 1:327925/139‑1 (MQ=255) ttGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTAACGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg < 2:507823/139‑1 (MQ=255) gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGcc > 2:444946/1‑129 (MQ=255) gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGcc < 1:444946/129‑1 (MQ=255) gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtgtg > 2:266257/1‑139 (MQ=255) ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCt > 1:642911/1‑139 (MQ=255) ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCt > 2:753650/1‑139 (MQ=255) gtgtTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTa > 2:662536/1‑139 (MQ=255) gTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGccc > 1:754347/1‑139 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGAt > 2:640938/1‑103 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGAt < 1:640938/103‑1 (MQ=255) aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaaa < 2:754347/139‑1 (MQ=255) gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAgcg > 2:112890/1‑88 (MQ=255) gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAgcg < 1:112890/88‑1 (MQ=255) gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCa < 2:8271/112‑1 (MQ=255) gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCa > 1:8271/1‑112 (MQ=255) gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAAt > 1:399901/1‑139 (MQ=255) aaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt > 1:356872/1‑109 (MQ=255) aaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt < 2:356872/109‑1 (MQ=255) aCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACaaa < 2:803414/139‑1 (MQ=255) tGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcagca < 1:753650/139‑1 (MQ=255) ttACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg < 2:399901/139‑1 (MQ=255) tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCAAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc < 2:172/139‑1 (MQ=255) ccGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTgc < 1:524100/82‑1 (MQ=255) ccGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTgc > 2:524100/1‑82 (MQ=255) ccGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑ggggg < 2:239371/54‑2 (MQ=255) ccGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑ggggg > 1:239371/1‑53 (MQ=255) gTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcagc < 1:278642/124‑1 (MQ=255) gTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcagc > 2:278642/1‑124 (MQ=255) gTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTATATGGTAAAGc > 2:420465/1‑139 (MQ=255) tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCt > 1:79503/1‑139 (MQ=255) gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATg < 2:532156/139‑1 (MQ=255) gTTCGATTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACtt < 2:79974/139‑1 (MQ=255) aTTCAGAACTGGAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCgg < 2:574885/139‑1 (MQ=255) gAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgt < 1:181080/53‑1 (MQ=255) gAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgt > 2:181080/1‑53 (MQ=255) gAAGCCATCGAAGGGGG‑CTGGAGCGCTCCTGTAAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTc > 1:400026/1‑139 (MQ=255) gAAGCCATCGAA‑GGGGACTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCa < 2:795509/117‑1 (MQ=255) gAAGCCATCGAA‑GGGGACTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCa > 1:795509/1‑117 (MQ=255) cATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg < 2:642911/139‑1 (MQ=255) cATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg < 1:266257/139‑1 (MQ=255) cATCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg < 2:79503/139‑1 (MQ=255) tCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCt > 1:726589/1‑139 (MQ=255) tCGAAGGGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCt > 1:84722/1‑139 (MQ=255) | TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑GGGG‑CTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCT > NC_000913/3896908‑3897179 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |