| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,980,957 | (G)6→7 | coding (71/1386 nt) | yifK → | putative APC family amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,980,951 | 1 | . | G | 96.0% | 172.2 / ‑0.8 | 50 | coding (65/1386 nt) | yifK | putative APC family amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (25/23); total (27/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCT‑GGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAT > NC_000913/3980836‑3981083 | aTAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGc < 2:52396/139‑1 (MQ=255)aTAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGc < 1:4055/139‑1 (MQ=255)aTAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGc < 1:774179/139‑1 (MQ=255) tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGcc > 2:359876/1‑139 (MQ=255) tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGcc < 1:50674/139‑1 (MQ=255) aCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCAACATATTGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTg > 2:478789/1‑139 (MQ=255) gATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGtt > 1:423301/1‑139 (MQ=255) tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTa > 2:268316/1‑139 (MQ=255) aCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATg < 2:423301/139‑1 (MQ=255) ggTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATgggg > 1:22287/1‑139 (MQ=255) tAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATgggg > 1:150272/1‑137 (MQ=255) tAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATgggg < 2:150272/137‑1 (MQ=255) aaCACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGgcc > 2:623466/1‑139 (MQ=255) aaCACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGgcc > 1:616926/1‑139 (MQ=255) ccGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa > 1:474933/1‑139 (MQ=255) ccGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 2:341261/139‑1 (MQ=255) ggAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCCG‑GGGGG‑GCCCCATTGGCGTCGGCCTGTTTATGAGGGCCGCCAGt > 1:109781/1‑139 (MQ=255) aaGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc < 2:616926/139‑1 (MQ=255) aaGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc > 1:204026/1‑139 (MQ=255) aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGt > 2:449711/1‑97 (MQ=255) aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGt < 1:449711/97‑1 (MQ=255) aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgg > 2:499504/1‑139 (MQ=255) cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc < 2:22287/139‑1 (MQ=255) cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGCCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc < 2:183377/139‑1 (MQ=255) tttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTa > 2:74945/1‑139 (MQ=255) tttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTa > 2:207361/1‑139 (MQ=255) tttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTa > 1:364945/1‑139 (MQ=255) aTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg < 2:204026/139‑1 (MQ=255) tGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt < 1:74945/139‑1 (MQ=255) ggCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCT‑ggggg‑gg > 2:586737/1‑69 (MQ=255) ggCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCT‑ggggg‑gg < 1:586737/70‑2 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc > 2:745821/1‑106 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc < 1:745821/106‑1 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc > 2:25939/1‑139 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc < 1:207361/139‑1 (MQ=255) agagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGc < 2:740066/71‑1 (MQ=255) agagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGc > 1:740066/1‑71 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc < 1:362059/109‑1 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCg > 1:455731/1‑139 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTGATGGGGGCCGCCAGTACCCTGAAATgggccgggc > 2:362059/1‑109 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCT‑ggggg‑gg > 1:72401/1‑52 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCT‑ggggg‑gg < 2:72401/53‑2 (MQ=255) cGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTc < 1:623466/139‑1 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGc > 1:645961/1‑59 (MQ=255) tGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGc < 2:645961/59‑1 (MQ=255) ggCTGGAGGCTCGACATATCGAACTCATTG‑CCCT‑GGGGGTGCACCATTGGCGTCGGCCTGTTGATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGGTCGTCt > 1:526330/1‑139 (MQ=255) gCTCGACATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatc < 1:752745/139‑1 (MQ=255) aCATATCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCg < 2:455731/139‑1 (MQ=255) atCGAACTCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCa > 1:502106/1‑139 (MQ=255) tCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatc > 1:383330/1‑123 (MQ=255) tCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatc < 2:383330/123‑1 (MQ=255) tCATCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCg < 1:499504/139‑1 (MQ=255) aTCG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGaa > 2:754216/1‑139 (MQ=255) cG‑CCCTGGGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAt > 2:15481/1‑139 (MQ=255) | ATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCG‑CCCT‑GGGGG‑GCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAT > NC_000913/3980836‑3981083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |