| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,287,217 | 0 | G | T | 50.0% | 4.2 / 48.3 | 28 | noncoding (20/171 nt) | rttR | rtT sRNA, processed from tyrT transcript |
| Reads supporting (aligned to +/- strand): ref base G (6/8); new base T (9/5); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTT > NC_000913/1287101‑1287345 | ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGaa > 2:573217/1‑138 (MQ=11)ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGaa < 1:573217/138‑1 (MQ=11) ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg < 1:117264/139‑1 (MQ=255) ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg < 2:903/139‑1 (MQ=255) ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg < 2:153044/139‑1 (MQ=255) ctctctCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGa > 2:364414/1‑112 (MQ=11) ctctctCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGa < 1:364414/112‑1 (MQ=11) tCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAggg > 2:786397/1‑83 (MQ=14) tCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAggg < 1:786397/83‑1 (MQ=14) cGAGACGAACCGTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATATGATTGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGc > 1:466155/1‑139 (MQ=11) aCCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc < 1:734265/139‑1 (MQ=14) aatCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggg < 2:459382/91‑1 (MQ=255) aatCGAAGCTTCTCATCATTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACGTTTGAAAGTGATGGTGGTggg > 1:459382/3‑93 (MQ=255) atCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg < 2:772571/138‑1 (MQ=14) aGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTccc > 1:42973/1‑139 (MQ=17) ctcATCCTTCCCCGCATTGGCAGAATATTTGATTGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGATCCCTTTgg > 1:399581/1‑139 (MQ=17) tCCCCGCATGGGCAGAATATTTGATTGCGGATTCG‑CTTG‑‑AG‑‑‑A‑GGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGc > 2:10182/1‑101 (MQ=14) tCCCCGCATGGGCAGAATATTTGATTGCGGATTCG‑CTTG‑‑AG‑‑‑A‑GGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGc < 1:10182/101‑1 (MQ=14) aTGGGCAGAATATTTGATTGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGc < 1:484993/125‑1 (MQ=18) aTGGGCAGAATATTTGATTGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATGCGAACCTTCGAAGTCGATGACGGCAGACTTACAGTCTGCTCCCTTTGGc > 2:484993/1‑125 (MQ=17) ggCAGAATATTTGATTGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCccacc > 2:264288/1‑139 (MQ=18) tttgaTTGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAc < 1:231752/120‑1 (MQ=17) tttGATTGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAc > 2:231752/1‑124 (MQ=17) tGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGAtg < 2:468586/41‑1 (MQ=255) tGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGAtg > 1:468586/1‑41 (MQ=255) tGCGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAt > 1:427418/1‑139 (MQ=255) cGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAttt > 2:455358/1‑139 (MQ=18) cGGATTCG‑CTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAttt > 1:737585/1‑139 (MQ=255) | TTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCG‑TTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTT > NC_000913/1287101‑1287345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |