Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	522,143	A→G	E576E (GAA→GAG)	ybbP →	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	522,143	0	A	G	100.0%	117.8 / NA	33	E576E (GAA→GAG)	ybbP	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/17);  total (16/17)

CAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTC  >  NC_000913/522019‑522272                                                                                                                             |                                                                                                                                  cAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGaa                                                                                                                     <  1:620929/139‑1 (MQ=255)           tGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAAc                                                                                                           >  2:547/1‑139 (MQ=255)             aaGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGt                                                                                                         <  1:566123/139‑1 (MQ=255)                 tGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                     >  1:581078/1‑139 (MQ=255)                       gcTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGt                                                                                               >  1:212346/1‑139 (MQ=255)                       gcTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGt                                                                                               >  2:413392/1‑139 (MQ=255)                        cTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTg                                                                                              <  2:233789/139‑1 (MQ=255)                        cTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTg                                                                                              >  1:502908/1‑139 (MQ=255)                         ttAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGa                                                                                             >  1:486203/1‑139 (MQ=255)                                cgAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTACCCTCGGTGATACCGTg                                                                                      <  2:502908/139‑1 (MQ=255)                                             aCCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCg                                                                         <  2:581078/139‑1 (MQ=255)                                             aCCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCg                                                                         <  2:659559/139‑1 (MQ=255)                                              ccTGGCAAAATACGCGACCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTG‑CCTCGGTGATACCGTGACTTTTATGGGCGAt                                                                       >  1:578714/1‑139 (MQ=255)                                                          cgcgGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGtt                                                            <  1:547/139‑1 (MQ=255)                                                          cgcgGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGtt                                                            <  2:212346/139‑1 (MQ=255)                                                             gacccGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTG‑CCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCcgc                                                        <  2:578714/137‑1 (MQ=255)                                                                   aTCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTaa                                                   >  2:711828/1‑139 (MQ=255)                                                                       tAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                     <  1:325096/85‑1 (MQ=255)                                                                       tAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGcc                                                                                                     >  2:325096/1‑85 (MQ=255)                                                                        aaTCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGa                                              <  1:253969/139‑1 (MQ=255)                                                                          tCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGAcc                                            <  1:320021/139‑1 (MQ=255)                                                                              aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCcgcg                                                       <  1:127403/124‑1 (MQ=255)                                                                              aTTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTCACTTTTATGGGCGATACCCAGGAGTTCcgcg                                                       >  2:127403/1‑124 (MQ=255)                                                                                    gCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTg                                                                                      >  1:24433/1‑87 (MQ=255)                                                                                    gCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTg                                                                                      <  2:24433/87‑1 (MQ=255)                                                                                              ggCCGCCAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTg                        >  1:629733/1‑139 (MQ=255)                                                                                                 cgccAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGa                     <  1:62698/139‑1 (MQ=255)                                                                                                   ccAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGAc                                             <  1:72254/113‑1 (MQ=255)                                                                                                   ccAAAAGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGAc                                             >  2:72254/1‑113 (MQ=255)                                                                                                       aaGCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCt               >  2:714735/1‑139 (MQ=255)                                                                                                         gCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACg                                                                                                          >  2:180575/1‑46 (MQ=255)                                                                                                         gCCGATGAAGTGTCGATGGAGGAGGGGCTGGCGAAACGCTTAAACg                                                                                                          <  1:180575/46‑1 (MQ=255)                                                                                                                    gtCGATGGAGGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTc  >  2:249586/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                  CAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGATCATAATCCGATTGTCGCCGGTAACTGGCCGCCAAAAGCCGATGAAGTGTCGATGGAAGAGGGGCTGGCGAAACGCTTAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTC  >  NC_000913/522019‑522272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑