Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 75,447 A→G C12R (TGC→CGC)  thiB ← thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091375,4470AG100.0% 122.7 / NA 35C12R (TGC→CGC) thiBthiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/14);  total (21/14)

CACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCA  >  NC_000913/75316‑75575
                                                                                                                                   |                                                                                                                                
caccaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcagcaac                                                                                                                           <  1:718552/139‑1 (MQ=255)
 accaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcagcaaca                                                                                                                          >  2:761802/1‑139 (MQ=255)
            ttCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGa                                                                                                               >  2:120491/1‑139 (MQ=255)
               gCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACAt                                                                                                            <  1:120491/139‑1 (MQ=255)
               gCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACAt                                                                                                            <  1:437841/139‑1 (MQ=255)
                           ggCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACt                                                                                                >  1:627587/1‑139 (MQ=255)
                             cTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACttt                                                                                              >  2:786194/1‑139 (MQ=255)
                                             aaCCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAagag                                                                              <  2:624169/139‑1 (MQ=255)
                                             aaCCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAagag                                                                              <  2:277316/139‑1 (MQ=255)
                                                   cGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCaa                                                                        >  1:450995/1‑139 (MQ=255)
                                                             ccccAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTgag                                                              >  2:766128/1‑139 (MQ=255)
                                                                 aGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAgg                                                          <  1:766128/139‑1 (MQ=255)
                                                                     ggcggcGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGcc                                                      >  1:380065/1‑139 (MQ=255)
                                                                                         tataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGt                                  >  2:139768/1‑139 (MQ=255)
                                                                                           taAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTa                                >  2:424404/1‑139 (MQ=255)
                                                                                                 gTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGa                          >  1:240161/1‑139 (MQ=255)
                                                                                                  tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                                                                                           <  1:479762/73‑1 (MQ=255)
                                                                                                  tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                                                                                           >  2:479762/1‑73 (MQ=255)
                                                                                                  tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAagag                                                                              >  1:505623/1‑86 (MQ=255)
                                                                                                  tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAagag                                                                              <  2:505623/86‑1 (MQ=255)
                                                                                                  tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGAt                         >  1:111974/1‑139 (MQ=255)
                                                                                                  tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGAt                         >  2:725861/1‑139 (MQ=255)
                                                                                                  tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGAt                         >  1:240597/1‑139 (MQ=255)
                                                                                                  tCAGAACGGGTTTAGCGAAAACGGGCGCTGCGCGCAGCAACAGCAGGGGAAGACATTTTTTCAACACTTTGCACCTCAACAAAGAGTGGCACAGGACTTGAGAAGGACGCGCAAATCCATGCGCCGGGGCTATCCGGAt                         >  1:729002/1‑139 (MQ=255)
                                                                                                   cAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                        >  2:302103/1‑139 (MQ=255)
                                                                                                      aaCGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAgg                     >  1:275901/1‑139 (MQ=255)
                                                                                                           gTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGa                <  1:761802/139‑1 (MQ=255)
                                                                                                           gTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGa                <  2:450995/139‑1 (MQ=255)
                                                                                                           gTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGa                <  1:56382/139‑1 (MQ=255)
                                                                                                            tttAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                                                                                           <  2:173050/63‑1 (MQ=255)
                                                                                                            tttAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                                                                                           >  1:173050/1‑63 (MQ=255)
                                                                                                            tttAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCg                                   >  2:473115/1‑119 (MQ=255)
                                                                                                            tttAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCg                                   <  1:473115/119‑1 (MQ=255)
                                                                                                                gCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGt           >  2:433859/1‑139 (MQ=255)
                                                                                                                     aaCGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCg                                      >  1:641579/1‑107 (MQ=255)
                                                                                                                     aaCGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCg                                      <  2:641579/107‑1 (MQ=255)
                                                                                                                         ggCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCa  <  2:111974/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                
CACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCA  >  NC_000913/75316‑75575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑