Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,029,450	C→T	V174I (GTC→ATC)	rlmI ←	23S rRNA m(5)C1962 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,029,450	0	C	T	100.0%	129.5 / NA	41	V174I (GTC→ATC)	rlmI	23S rRNA m(5)C1962 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/23);  total (18/23)

GCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCA  >  NC_000913/1029319‑1029580                                                                                                                                    |                                                                                                                                   gcggcgGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGccc                                                                                                                             >  2:107650/1‑139 (MQ=255)                  ggcTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCCTCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACGCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTcc                                                                                                            <  2:603882/139‑1 (MQ=255)                      aTCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCcttct                                                                                                        <  1:65970/139‑1 (MQ=255)                              ggTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACg                                                                                                <  2:94785/139‑1 (MQ=255)                               gTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGt                                                                                               >  1:745589/1‑139 (MQ=255)                                     gtagtaGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACcgcg                                                                                         <  2:614855/139‑1 (MQ=255)                                         tagCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTa                                                                                                  <  2:112428/126‑1 (MQ=255)                                         tagCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTa                                                                                                  >  1:112428/1‑126 (MQ=255)                                         tagCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGt                                                                                     >  1:373104/1‑139 (MQ=255)                                         tagCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGt                                                                                     >  2:692189/1‑139 (MQ=255)                                         tagCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGt                                                                                     >  2:450027/1‑139 (MQ=255)                                          agCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTc                                                                                    >  2:27465/1‑139 (MQ=255)                                            cccGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGc                                                                                  >  2:202891/1‑139 (MQ=255)                                              cGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTg                                                                                >  2:414819/1‑139 (MQ=255)                                              cGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTg                                                                                >  2:476628/1‑139 (MQ=255)                                                   tgtgTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTcc                                                                                                            <  1:74132/106‑1 (MQ=255)                                                   tgtgTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATc                                                                           <  1:450027/139‑1 (MQ=255)                                                   tgtgTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATc                                                                           <  1:476628/139‑1 (MQ=255)                                                   tgtgTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATc                                                                           <  1:242153/139‑1 (MQ=255)                                                   tgtgTCCGTGCTGAATATCCACCAGCAGCTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGCCCCTGGGTCAGCTACATTcc                                                                                                            >  2:74132/1‑106 (MQ=255)                                                       tCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTaa                                                                       >  2:457965/1‑139 (MQ=255)                                                           tGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACcgc                                                                                          >  1:143544/1‑116 (MQ=255)                                                           tGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACcgc                                                                                          <  2:143544/116‑1 (MQ=255)                                                           tGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCg                                                                   >  2:733950/1‑139 (MQ=255)                                                           tGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCg                                                                   <  1:692189/139‑1 (MQ=255)                                                           tGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCg                                                                   >  1:431211/1‑139 (MQ=255)                                                            gCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGa                                                                  <  1:540115/139‑1 (MQ=255)                                                                       accaGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGTTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGt                                                       <  1:518704/139‑1 (MQ=255)                                                                       accaGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGt                                                       <  1:457965/139‑1 (MQ=255)                                                                       accaGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGt                                                       <  1:107650/139‑1 (MQ=255)                                                                          agcagTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGt                                                       >  1:478244/1‑136 (MQ=255)                                                                          agcagTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGt                                                       <  2:478244/136‑1 (MQ=255)                                                                                   aTTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCa                                           <  2:13861/139‑1 (MQ=255)                                                                                   aTTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCa                                           <  2:373104/139‑1 (MQ=255)                                                                                      ccGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAggg                                        <  2:431211/139‑1 (MQ=255)                                                                                                       cagcagGGCAGGTGGCAACTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCcgcg                       <  1:698123/139‑1 (MQ=255)                                                                                                                        aCTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCa                                           <  2:40269/102‑1 (MQ=255)                                                                                                                        aCTCGCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCa                                           >  1:40269/1‑102 (MQ=255)                                                                                                                            gCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTc            >  2:440382/1‑129 (MQ=255)                                                                                                                            gCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTc            <  1:440382/129‑1 (MQ=255)                                                                                                                            gCCGGTGATGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCa  <  1:740619/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                   GCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCATTCCGTGTTCTTCAATCGGCAGCAGGGCAGGTGGCAACTCGCCGGTGACGGGGCCCTGGGTCAGCTCCATTCCTTCTTTTTTACGTACCGCGACGTCGCTGCGATCGTAAATCGAACATTCCGGGTACAGCGTTTGCAGGGCACTAATTAATGCCGCGCGCTGATATTCTGCCCCAGCA  >  NC_000913/1029319‑1029580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑