Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,264,970	G→A	*419* (TAG→TAA)	hemA →	glutamyl tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,264,970	0	G	A	100.0%	110.0 / NA	35	*419* (TAG→TAA)	hemA	glutamyl tRNA reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/16);  total (19/16)

GATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGA  >  NC_000913/1264848‑1265103                                                                                                                           |                                                                                                                                      gATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCt                                                                                                                       >  1:183276/1‑139 (MQ=255) gATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCt                                                                                                                       >  1:336701/1‑139 (MQ=255)   tCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCttt                                                                                                                     <  1:547066/139‑1 (MQ=255)      ggCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCtttttt                                                                                                                  >  1:22035/1‑139 (MQ=255)           ggAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAg                                                                                                             >  2:730372/1‑139 (MQ=255)           ggAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAg                                                                                                             <  1:556186/139‑1 (MQ=255)            gAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAgg                                                                                                            >  1:548319/1‑139 (MQ=255)               aCTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTg                                                                                                         <  2:336701/139‑1 (MQ=255)                 tGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGga                                                                                                       >  2:284981‑M1/1‑137 (MQ=255)                 tGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGga                                                                                                       >  2:445172‑M1/1‑137 (MQ=255)                   aCTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg                                                                                                     <  1:785804‑M1/139‑5 (MQ=255)                            ttGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataat                                                                                            <  2:39068‑M1/139‑14 (MQ=255)                              gATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatgg                                                                                          <  2:548319‑M1/139‑16 (MQ=255)                                  cATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaata                                                                                      <  1:284981‑M1/139‑20 (MQ=255)                                   aTGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatat                                                                                     >  1:92942‑M1/1‑119 (MQ=255)                                    tGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatc                                                                                    >  2:706237‑M1/1‑118 (MQ=255)                                                             gccgccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataag                                                           <  1:706237‑M1/139‑47 (MQ=255)                                                             gccgccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataag                                                           <  1:452903‑M1/139‑47 (MQ=255)                                                               cgccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagcc                                                         >  2:316704‑M1/1‑91 (MQ=255)                                                                 cccGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg                                                                                                     <  2:162631‑M1/93‑5 (MQ=255)                                                                 cccGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg                                                                                                     >  1:162631‑M1/1‑89 (MQ=255)                                                                        cGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa                                                                                                                >  2:216621/1‑75 (MQ=255)                                                                        cGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa                                                                                                                <  1:216621/75‑1 (MQ=255)                                                                            gATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaac                                                      <  2:59238‑M1/129‑52 (MQ=255)                                                                            gATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaac                                                      >  1:59238‑M1/1‑78 (MQ=255)                                                                               aacgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcga                                         >  1:131880‑M1/1‑75 (MQ=255)                                                                                                  tGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa                                                                                                                <  2:88431/49‑1 (MQ=255)                                                                                                  tGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa                                                                                                                >  1:88431/1‑49 (MQ=255)                                                                                                  tGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgct                      >  1:508256‑M1/1‑56 (MQ=255)                                                                                                     gcgACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGt                                                                                                          <  1:740395/52‑1 (MQ=255)                                                                                                     gcgACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGt                                                                                                          >  2:740395/1‑52 (MQ=255)                                                                                                            ccTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaaatcctgc            <  1:730372‑M1/139‑94 (MQ=255)                                                                                                                      gAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaaatcctgctggagcatgt  >  1:580203‑M1/1‑36 (MQ=255)                                                                                                                      gAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatgg                                                                                          >  2:443195‑M1/1‑36 (MQ=255)                                                                                                                      gAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatgg                                                                                          <  1:443195‑M1/51‑16 (MQ=255)                                                                                                                           |                                                                                                                                      GATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGA  >  NC_000913/1264848‑1265103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑