Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,340,525 C→T P95S (CCG→TCG)  yciS → DUF1049 family inner membrane protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,340,5250CT100.0% 57.4 / NA 19P95S (CCG→TCG) yciSDUF1049 family inner membrane protein, function unknown
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/8);  total (11/8)

TGCTGCGGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGGCGCGCGCTGAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGCCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCGCTGCCTATGGCTGGTATATGGGCCGCAGAAGTGCGCAACAAAACAAGCAAGATGAAGCCAAC  >  NC_000913/1340389‑1340656
                                                                                                                                        |                                                                                                                                   
tgctgcGGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGGCGCGCGCTGAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTCg                                                                                                                                   >  2:420569/1‑139 (MQ=255)
              cTATCGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGGCGCGCGCTGAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTCGCACTCGTCAgcggc                                                                                                                     <  2:83770/139‑1 (MQ=255)
                     ttggttgATTTGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGGCGCGCGCTGAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTCGCACTCGTCAGCGGCGAAGGaa                                                                                                              <  1:413614/139‑1 (MQ=255)
                          tgATTTGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGGCGCGCGCTGAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTCGCACTCGTCAGCGGCGAAGGAATAACt                                                                                                         <  2:468732/139‑1 (MQ=255)
                             tttGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGGCGCGCGCTGAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTCGCACTCGTCAGCGGCGAAg                                                                                                                 >  1:393942/1‑128 (MQ=255)
                             tttGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGGCGCGCGCTGAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTCGCACTCGTCAGCGGCGAAg                                                                                                                 <  2:393942/128‑1 (MQ=255)
                                                                        cgcTGAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTGGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCGCTGcc                                                           >  1:12756/1‑139 (MQ=255)
                                                                        cgcTGAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCGCTGcc                                                           >  2:99185/1‑139 (MQ=255)
                                                                                     aTAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAgttg                                                                                         <  1:592939/96‑1 (MQ=255)
                                                                                     aTAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAgttg                                                                                         >  2:592939/1‑96 (MQ=255)
                                                                                     aTAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCGCTGCCTATGGCTGGtata                                              <  1:161419/139‑1 (MQ=255)
                                                                                       aaaGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCGCTGCCTATGGCTGGTATATg                                            <  1:99185/139‑1 (MQ=255)
                                                                                       aaaGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCGCTGCCTATGGCTGGTATATg                                            >  2:667808/1‑139 (MQ=255)
                                                                                         aGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCGCTGCCTATGGCTGGTATATggg                                          >  1:437153/1‑139 (MQ=255)
                                                                                            gACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCg                                                                >  2:326240/1‑114 (MQ=255)
                                                                                            gACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGTCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCg                                                                <  1:326240/114‑1 (MQ=255)
                                                                                                            ttCACCCGCGACTGACGTGGCTGTAGTGTCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCGCTGCCTATGGCTGGTATATGGGCCGCAGAAGTGCGcaacaa                       >  2:291703/1‑139 (MQ=255)
                                                                                                                           cGTGGCTGTAGTGTCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCGCTGCCTATGGCTGGTATATGGGCCGCAGAAGTGCGCAACAAAACAAGCAAGATGaa        >  2:794205/1‑139 (MQ=255)
                                                                                                                                 tGTAGTGTCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCGCTGCCTATGGCTGGTATATGGGCCGCAGAAGTGCGCAACAAAACAAGCAAGATGAAGCCAAc  >  2:711057/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                   
TGCTGCGGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGGCGCGCGCTGAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGCCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCGCTGCCTATGGCTGGTATATGGGCCGCAGAAGTGCGCAACAAAACAAGCAAGATGAAGCCAAC  >  NC_000913/1340389‑1340656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: