Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,354,505 C→T S5N (AGC→AAC) 
*322* (TAG→TAA
sapC ←
sapB ←
antimicrobial peptide transport ABC transporter permease
antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,354,5050CT100.0% 66.8 / NA 23S5N (AGC→AAC) 
*322* (TAG→TAA
sapC
sapB
antimicrobial peptide transport ABC transporter permease
antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/14);  total (9/14)

CGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGC  >  NC_000913/1354369‑1354637
                                                                                                                                        |                                                                                                                                     
cgcAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTa                                                                                                                                     >  2:299861/1‑139 (MQ=255)
                       acaGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTAt                                                                                                              <  1:690549/139‑1 (MQ=255)
                        caGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATg                                                                                                             <  1:411959/139‑1 (MQ=255)
                               gccagccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCaga                                                                                                      <  1:364178/139‑1 (MQ=255)
                                   gccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATAcc                                                                                                                       <  2:783394/118‑1 (MQ=255)
                                   gccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATAcc                                                                                                                       >  1:783394/1‑118 (MQ=255)
                                                    aGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCaaa                                                                                <  2:548939/139‑1 (MQ=255)
                                                           ccATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAg                                                                         >  1:79633/1‑139 (MQ=255)
                                                                gCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTc                                                                                                         <  1:320078/103‑1 (MQ=255)
                                                                gCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTc                                                                                                         >  2:320078/1‑103 (MQ=255)
                                                                     ggCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGtt                                                               <  2:287191/139‑1 (MQ=255)
                                                                         tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAg                                                                                                                              <  2:465745/73‑1 (MQ=255)
                                                                         tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAg                                                                                                                              >  1:465745/1‑73 (MQ=255)
                                                                         tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACa                                                           >  1:663993/1‑139 (MQ=255)
                                                                                     ttGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGt                                               <  1:752038/139‑1 (MQ=255)
                                                                                     ttGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGt                                               <  2:79633/139‑1 (MQ=255)
                                                                                       gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGa                                             >  1:94853/1‑139 (MQ=255)
                                                                                        cgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAg                                            <  1:158742/139‑1 (MQ=255)
                                                                                                            gCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTcc                                                                                                                  <  2:23971/50‑1 (MQ=255)
                                                                                                            gCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTcc                                                                                                                  >  1:23971/1‑50 (MQ=255)
                                                                                                                    ggcgCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATgg                <  2:94853/139‑1 (MQ=255)
                                                                                                                             cGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGGTTAGCCATGGCACCCAATATATCAGAAATCACGTTAACAATAATGACCAGTGAGTCACACACCATCACTCCGGCGGAAATGGCTGCGTaa        <  1:299861/139‑1 (MQ=255)
                                                                                                                                  tataCGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCctgc  >  1:219376/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                     
CGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGGCTGCGTAATCCTGC  >  NC_000913/1354369‑1354637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: