Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,450,392	C→T	A320T (GCA→ACA)	tynA ←	tyramine oxidase, copper‑requiring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,450,392	0	C	T	100.0%	110.2 / NA	35	A320T (GCA→ACA)	tynA	tyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/19);  total (16/19)

CATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGA  >  NC_000913/1450258‑1450526                                                                                                                                       |                                                                                                                                       cATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTc                                                                                                                                    >  1:439150/1‑139 (MQ=255)   tGCTGAGGTTAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCAt                                                                                                                                  <  2:514818/139‑1 (MQ=255)        aGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCa                                                                                                                             >  2:441418/1‑139 (MQ=255)                       ttCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGAc                                                                                                              <  1:441418/139‑1 (MQ=255)                         ccGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACct                                                                                                            >  2:453156/1‑139 (MQ=255)                            ccAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACcttct                                                                                                         >  1:267689/1‑139 (MQ=255)                                            ccAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGa                                                                                                               >  1:84450/1‑117 (MQ=255)                                            ccAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGa                                                                                                               <  2:84450/117‑1 (MQ=255)                                            ccAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGa                                                                                                               <  2:709838/117‑1 (MQ=255)                                            ccAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGa                                                                                                               >  1:709838/1‑117 (MQ=255)                                                 aaTGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTc                                                                                    <  2:267689/139‑1 (MQ=255)                                                     gtgtAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGtt                                                                                <  1:48255/139‑1 (MQ=255)                                                      tgtAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTc                                                                               >  1:119407/1‑139 (MQ=255)                                                        tAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTa                                                                             <  1:591942/139‑1 (MQ=255)                                                          aTTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGaa                                                                                                                       >  1:387786/1‑95 (MQ=255)                                                          aTTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGaa                                                                                                                       <  2:387786/95‑1 (MQ=255)                                                              ttACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCaa                                                                       <  2:439150/139‑1 (MQ=255)                                                                aCCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGTAACTACCGGACCTTCTTCAACCTTAACGATTTTTTTCTGTTCTAAATCAACg                                                                     >  2:652332/1‑139 (MQ=255)                                                                            aaTGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGa                                                                                                                        >  1:229099/1‑76 (MQ=255)                                                                            aaTGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGa                                                                                                                        <  2:229099/76‑1 (MQ=255)                                                                                       tAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATggg                                              <  1:62421/139‑1 (MQ=255)                                                                                             tAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGc                                        <  1:453156/139‑1 (MQ=255)                                                                                               aCTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATggg                                              <  2:308774/131‑1 (MQ=255)                                                                                               aCTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATggg                                              >  1:308774/1‑131 (MQ=255)                                                                                               aCTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGccc                                      >  1:374304/1‑139 (MQ=255)                                                                                                    cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGAc                                                                                                              <  2:139913/62‑1 (MQ=255)                                                                                                    cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGAc                                                                                                              >  1:139913/1‑62 (MQ=255)                                                                                                    cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTaa                                                                                               <  2:237033/77‑1 (MQ=255)                                                                                                    cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTaa                                                                                               >  1:237033/1‑77 (MQ=255)                                                                                                                  cACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGAc                   <  2:374304/139‑1 (MQ=255)                                                                                                                           cAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGccacc                                                            <  1:685029/89‑1 (MQ=255)                                                                                                                           cAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGccacc                                                            >  2:685029/1‑89 (MQ=255)                                                                                                                                 ggCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCtgat    >  2:721906/1‑139 (MQ=255)                                                                                                                                   cGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCGGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCtgatga  <  2:119407/139‑1 (MQ=255)                                                                                                                                   cGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCtgatga  <  1:652332/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       CATGCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGA  >  NC_000913/1450258‑1450526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑