Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	125,227	T→C	R737R (CGT→CGC)	aceE →	pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	125,227	0	T	C	100.0%	67.8 / NA	20	R737R (CGT→CGC)	aceE	pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/12);  total (8/12)

AACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGTCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGC  >  NC_000913/125096‑125338                                                                                                                                    |                                                                                                                aaCGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTAC‑AACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCg                                                                                                         <  1:453870/139‑1 (MQ=255)     aaaaCTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGa                                                                                                      <  1:9326/139‑1 (MQ=255)            ccACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCt                                                                                               <  2:287600/139‑1 (MQ=255)            ccACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCt                                                                                               <  2:28491/139‑1 (MQ=255)                         aTGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGa                                                                                  >  2:594314/1‑139 (MQ=255)                                         aGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAgg                                                                  <  1:757878/139‑1 (MQ=255)                                           gAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGtt                                                                <  1:234108/139‑1 (MQ=255)                                               gTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGa                                                            <  1:98562/139‑1 (MQ=255)                                                     gTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTta                                                      >  2:759657/1‑139 (MQ=255)                                                          ggTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCg                                                 <  1:786307/139‑1 (MQ=255)                                                          ggTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCg                                                 >  1:209683/1‑139 (MQ=255)                                                            tATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTg                                               <  2:29893/139‑1 (MQ=255)                                                              tCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGAc                                             >  1:247034/1‑139 (MQ=255)                                                               cTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGAcc                                            >  2:151593/1‑139 (MQ=255)                                                                           aaCTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAg                                <  1:337947/139‑1 (MQ=255)                                                                                        aGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATg                   >  1:218535/1‑139 (MQ=255)                                                                                          cAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGt                 <  2:209683/139‑1 (MQ=255)                                                                                                         gctgctCGGCTCCGGTTCTATCCTGCACCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGc  >  1:169492/1‑139 (MQ=255)                                                                                                                  cTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGtt                                                                <  1:576748/68‑1 (MQ=255)                                                                                                                  cTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGtt                                                                >  2:576748/1‑68 (MQ=255)                                                                                                                                    |                                                                                                                AACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGTCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTGAACGC  >  NC_000913/125096‑125338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑