Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,575,652	A→G	G656G (GGT→GGC)	yddB ←	putative TonB‑dependent outer membrane receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,575,652	0	A	G	100.0%	234.3 / NA	66	G656G (GGT→GGC)	yddB	putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/36);  total (30/36)

TTACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGT  >  NC_000913/1575518‑1575784                                                                                                                                       |                                                                                                                                     ttACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGt                                                                                                                                  <  2:321328/139‑1 (MQ=255)   aCGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTta                                                                                                                                >  1:193499/1‑139 (MQ=255)   aCGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTta                                                                                                                                >  1:42572/1‑139 (MQ=255)                   gccagCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTAt                                                                                                                <  2:202557/139‑1 (MQ=255)                      agCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCAc                                                                                                             <  2:607635/139‑1 (MQ=255)                        cGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCg                                                                                                           >  2:262787/1‑139 (MQ=255)                           aTTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTa                                                                                                        <  1:142093/139‑1 (MQ=255)                             tCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTAtt                                                                                                      >  2:562465/1‑139 (MQ=255)                              cGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTg                                                                                                     <  1:753424/139‑1 (MQ=255)                                 ccACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGctc                                                                                                  <  1:516087/139‑1 (MQ=255)                                 ccACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGctc                                                                                                  >  2:184207/1‑139 (MQ=255)                                 ccACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGctc                                                                                                  <  2:115348/139‑1 (MQ=255)                                  cacaCTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGctct                                                                                                 <  2:273637/139‑1 (MQ=255)                                   acacTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCtctc                                                                                                <  1:92257/139‑1 (MQ=255)                                    cacTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTctcc                                                                                               >  2:737269/1‑139 (MQ=255)                                     acTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTctcct                                                                                              <  1:624622/139‑1 (MQ=255)                                           ccGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAAc                                                                                        <  2:639595/139‑1 (MQ=255)                                             gCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCa                                                                                      <  1:184207/139‑1 (MQ=255)                                              cTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCAt                                                                                     <  1:146756/139‑1 (MQ=255)                                               tCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCAtt                                                                                    >  2:233756/1‑139 (MQ=255)                                                           tGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACaa                                                                        >  1:764097/1‑139 (MQ=255)                                                              aaTCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGtt                                                                     <  1:784840/139‑1 (MQ=255)                                                                 ccATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGcc                                                                  >  2:402790/1‑139 (MQ=255)                                                                  cATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGccc                                                                 <  1:562465/139‑1 (MQ=255)                                                                  cATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGccc                                                                 <  2:685600/139‑1 (MQ=255)                                                                    tGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTg                                                                                                     >  1:516823/1‑101 (MQ=255)                                                                    tGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTg                                                                                                     <  2:516823/101‑1 (MQ=255)                                                                    tGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCtt                                                               <  2:576959/139‑1 (MQ=255)                                                                     gTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGt                                                                      <  1:5254/131‑1 (MQ=255)                                                                     gTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGt                                                                      >  2:5254/1‑131 (MQ=255)                                                                       ttAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGct                                                            <  2:262303/139‑1 (MQ=255)                                                                       ttAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGct                                                            <  2:132556/139‑1 (MQ=255)                                                                              gaTCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGata                                                     <  1:737269/139‑1 (MQ=255)                                                                               aTCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGatat                                                    >  1:93386/1‑139 (MQ=255)                                                                                tCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGctc                                                                                                  >  1:797253/1‑92 (MQ=255)                                                                                tCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGctc                                                                                                  <  2:797253/92‑1 (MQ=255)                                                                                tCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGAtata                                                   >  2:733993/1‑139 (MQ=255)                                                                                tCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGAtata                                                   >  2:355037/1‑139 (MQ=255)                                                                                        gggTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGa                                           <  1:733993/139‑1 (MQ=255)                                                                                           ttattaAAATCTGCCACTGGAACGCTATCGTAAGAGACCGGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCa                                                                                      <  1:591445/93‑1 (MQ=255)                                                                                           ttattaAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATATCCATTGTTTAACGACAAGTTGCCCTTGCTCTTGata                                                     <  2:98281/126‑1 (MQ=255)                                                                                           ttattaAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGccc                                                                 <  2:454367/114‑1 (MQ=255)                                                                                           ttattaAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGccc                                                                 >  1:454367/1‑114 (MQ=255)                                                                                           ttattaAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGata                                                     >  1:98281/1‑126 (MQ=255)                                                                                           ttattaAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACaa                                        >  1:417202/1‑139 (MQ=255)                                                                                           ttattaAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACaa                                        >  1:39250/1‑139 (MQ=255)                                                                                           ttattaAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCAACGGTATTGCTCTCCTCATAACCa                                                                                      >  2:591445/1‑93 (MQ=255)                                                                                            tattaAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAAc                                                                                        >  2:306007/1‑90 (MQ=255)                                                                                            tattaAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAAc                                                                                        <  1:306007/90‑1 (MQ=255)                                                                                                   aTCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTggg                                >  2:677682/1‑139 (MQ=255)                                                                                                    tCTGCCACTGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGt                               <  2:93386/139‑1 (MQ=255)                                                                                                            tGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGTTAAAGACAATTTGTGGGt                               <  1:445697/131‑1 (MQ=255)                                                                                                            tGGAACGCTATCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGt                               >  2:445697/1‑131 (MQ=255)                                                                                                                     aTCGTAAGAGACCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCgg              <  1:677682/139‑1 (MQ=255)                                                                                                                            gagaCCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCa       <  1:233756/139‑1 (MQ=255)                                                                                                                            gagaCCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCa       <  1:355037/139‑1 (MQ=255)                                                                                                                              gaCCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGctct                                                          <  1:220318/86‑1 (MQ=255)                                                                                                                              gaCCAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGctct                                                          >  2:220318/1‑86 (MQ=255)                                                                                                                                ccAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCa       <  1:244547/135‑1 (MQ=255)                                                                                                                                ccAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCa       >  2:244547/1‑135 (MQ=255)                                                                                                                                ccAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAgggg   >  1:477393/1‑139 (MQ=255)                                                                                                                                ccAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAgggg   >  1:513904/1‑139 (MQ=255)                                                                                                                                ccAGATTGCCGTTATAAACCACCTGGTAATCACCTGTATAGCTCTCCTCATAACCAGTGTTTAACGACAAGATGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAgggg   >  2:138336/1‑139 (MQ=255)                                                                                                                                 cAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTtg                                    >  1:377133/1‑105 (MQ=255)                                                                                                                                 cAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTtg                                    <  2:377133/105‑1 (MQ=255)                                                                                                                                 cAGATTGCCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGt  >  1:408661/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                     TTACGCGCTTCTTGCCAGGCCAGCGTATTCGCCCACACTAACCCGCTCGGTTGATGCGTGAAATCCATGTTTAAGGAGATCTTTAATGGGTTATTAAAATCTGCCACTGGAACGCTATCGTAAGAGACCAGATTACCGTTATAAACCACCTGGTTATCACCGGTATTGCTCTCCTCATAACCATTGTTTAACGACAAGTTGCCCTTGCTCTTGATATAGCTAAAGACAATTTGTGGGTTAATATCTACCTGGCGGATATGCAGGGGT  >  NC_000913/1575518‑1575784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑