Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,744,786 C→T Q444* (CAA→TAA)  mdtK → multidrug efflux system transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,744,7860CT100.0% 50.9 / NA 17Q444* (CAA→TAA) mdtKmultidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/5);  total (12/5)

CTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAAT  >  NC_000913/1744665‑1744919
                                                                                                                         |                                                                                                                                     
cTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGTAACGTCTGCCGTCAGcc                                                                                                                      >  1:627571/1‑139 (MQ=255)
             cTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAAc                                                                                                         >  1:412034/1‑139 (MQ=255)
                       tGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCg                                                                                               <  1:769810/139‑1 (MQ=255)
                                                                tttATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTa                                                      >  1:81029/1‑139 (MQ=255)
                                                                         ggtcTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCg                                                                                               <  1:225981/86‑1 (MQ=255)
                                                                         ggtcTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCg                                                                                               >  2:225981/4‑89 (MQ=255)
                                                                               aCGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCAt                                                                                                   >  1:566411/1‑79 (MQ=255)
                                                                               aCGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCAt                                                                                                   <  2:566411/79‑1 (MQ=255)
                                                                                                   gatgTTGCGTATGCGGTTCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCg                                                                                               >  2:184428/1‑63 (MQ=255)
                                                                                                   gatgTTGCGTATGCGGTTCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCg                                                                                               <  1:184428/63‑1 (MQ=255)
                                                                                                      gTTGCGTATGCGGTTCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCtt                >  1:689070/1‑139 (MQ=255)
                                                                                                      gTTGCGTATGCGGTTCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCtt                >  1:253478/1‑139 (MQ=255)
                                                                                                          cGTATGCGGTTCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTc            >  1:336803/1‑139 (MQ=255)
                                                                                                          cGTATGCGGTTCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTc            >  2:778191/1‑139 (MQ=255)
                                                                                                            tATGCGGTTCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTg                                                        >  1:789316/1‑93 (MQ=255)
                                                                                                            tATGCGGTTCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTg                                                        <  2:789316/93‑1 (MQ=255)
                                                                                                                    tCCTGTAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAAt  >  2:231053/1‑139 (MQ=255)
                                                                                                                         |                                                                                                                                     
CTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAAT  >  NC_000913/1744665‑1744919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: