Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,841,897 G→A *136* (TAG→TAA
T80I (ACT→ATT) 
nudG →
ynjH ←
CTP pyrophosphohydrolase, also hydrolyzes 2‑hydroxy‑dATP, 8‑hydroxy‑dGTP, 5‑hydroxy‑CTP, dCTP and 5‑methyl‑dCTP
DUF1496 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,841,8970GA100.0% 126.1 / NA 40*136* (TAG→TAA
T80I (ACT→ATT) 
nudG
ynjH
CTP pyrophosphohydrolase, also hydrolyzes 2‑hydroxy‑dATP, 8‑hydroxy‑dGTP, 5‑hydroxy‑CTP, dCTP and 5‑methyl‑dCTP
DUF1496 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/21);  total (19/21)

CGTTACAGGCACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTG  >  NC_000913/1841764‑1842031
                                                                                                                                     |                                                                                                                                      
cGTTACAGGCACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTa                                                                                                                                   <  2:748249/139‑1 (MQ=255)
             tGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtcat                                                                                                                      >  2:581079/1‑139 (MQ=255)
                aCATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCAc                                                                                                                   <  1:277236/139‑1 (MQ=255)
                aCATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCAc                                                                                                                   <  1:646928/139‑1 (MQ=255)
                            ggtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgca                                                                                                       >  1:314380/1‑139 (MQ=255)
                             gtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgcag                                                                                                      <  2:314380/139‑1 (MQ=255)
                             gtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgcag                                                                                                      <  1:266733/139‑1 (MQ=255)
                                  gtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTa                                                                                                 >  1:774093/1‑139 (MQ=255)
                                                    gcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTa                                                                               >  2:711902/1‑139 (MQ=255)
                                                        cAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGc                                                                           <  2:128609/139‑1 (MQ=255)
                                                                   ggCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGaat                                                                >  2:341083/1‑139 (MQ=255)
                                                                             gACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATc                                                      <  2:776809/139‑1 (MQ=255)
                                                                                aTTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCg                                                                            <  1:534554/114‑1 (MQ=255)
                                                                                aTTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCg                                                                            >  2:534554/1‑114 (MQ=255)
                                                                                  tCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGAt                                                 >  2:52555/1‑139 (MQ=255)
                                                                                   ccATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATa                                                >  1:103531/1‑139 (MQ=255)
                                                                                     attattAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATaaa                                              >  2:533812/1‑139 (MQ=255)
                                                                                       tattaGAGGCGTTGATGGCTTTACGCGCCGCCAGACCAGCCGATTAATGCGAAAGGTTTTGACATCACGCTGGCATTGCAGCAGTATTCATTCGGCTTTAATTACCGCCCTTTCAGAATAATTTTAATCCTGATAAACg                                            <  1:578375/139‑1 (MQ=255)
                                                                                         ttaGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgca                                          <  1:52555/139‑1 (MQ=255)
                                                                                            gagGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtc                                                                                                                        <  1:45449/58‑1 (MQ=255)
                                                                                            gagGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGtc                                                                                                                        >  2:45449/1‑58 (MQ=255)
                                                                                             agGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATc                                                      <  1:666247/123‑1 (MQ=255)
                                                                                             agGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATc                                                      >  2:666247/1‑123 (MQ=255)
                                                                                             agGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCAc                                      >  1:228979/1‑139 (MQ=255)
                                                                                               gCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTg                                    >  1:730717/1‑139 (MQ=255)
                                                                                                   ttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCa                                                                                                            <  1:801384/63‑1 (MQ=255)
                                                                                                   ttATGGCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCa                                                                                                            >  2:801384/1‑63 (MQ=255)
                                                                                                       ggCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAgg                            <  2:732134/139‑1 (MQ=255)
                                                                                                        gCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAggg                           <  1:341083/139‑1 (MQ=255)
                                                                                                        gCTTTACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAggg                           <  2:730717/139‑1 (MQ=255)
                                                                                                            tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgcag                                                                                                      >  1:780095/1‑60 (MQ=255)
                                                                                                            tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgcag                                                                                                      <  2:780095/60‑1 (MQ=255)
                                                                                                            tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgc                                                                                                        >  2:326584/1‑58 (MQ=255)
                                                                                                            tACGCGCCGCCAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTgc                                                                                                        <  1:326584/58‑1 (MQ=255)
                                                                                                                   cgccAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCGGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATTATTTTGATCCTGATAAACGCAGCACTGAGTa                                <  1:103750/123‑1 (MQ=255)
                                                                                                                   cgccAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTa                                >  2:103750/1‑123 (MQ=255)
                                                                                                                   cgccAGACCAGCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACt                >  1:433546/1‑139 (MQ=255)
                                                                                                                             gCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCg                                                                                         <  2:264952/56‑1 (MQ=255)
                                                                                                                             gCGGATTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCg                                                                                         >  1:264952/1‑56 (MQ=255)
                                                                                                                                 aTTAATGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaactg  <  1:421490/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
CGTTACAGGCACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTG  >  NC_000913/1841764‑1842031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: