Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,084,773	G→A	G251G (GGC→GGT)	yeeE ←	UPF0394 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,084,773	0	G	A	100.0%	147.3 / NA	47	G251G (GGC→GGT)	yeeE	UPF0394 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/25);  total (22/25)

TGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGAT  >  NC_000913/2084637‑2084907                                                                                                                                         |                                                                                                                                       tGGCACCGATGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATTAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  1:474040/139‑1 (MQ=255) tGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  1:387509/139‑1 (MQ=255)  ggCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAccc                                                                                                                                     <  2:770392/139‑1 (MQ=255)        gAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTa                                                                                                                               >  2:420641/1‑139 (MQ=255)         aaGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTaa                                                                                                                              >  1:107147/1‑139 (MQ=255)         aaGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTaa                                                                                                                              <  2:647783/139‑1 (MQ=255)          aGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAAt                                                                                                                             <  1:420641/139‑1 (MQ=255)                tCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTAttt                                                                                                                       <  2:107147/139‑1 (MQ=255)                         caccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCAcc                                                                                                              >  1:675321/1‑139 (MQ=255)                           ccTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACAcc                                                                                                                                      <  1:225349/113‑1 (MQ=255)                            cTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCcgc                                                                                                           >  1:292114/1‑139 (MQ=255)                              aaCCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGa                                                                                                         >  1:120783/1‑139 (MQ=255)                                 ccGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCa                                                                                                      <  2:162614/139‑1 (MQ=255)                                     tACGTAATGTTGTTTGTGCATCAGTTGCGCGAACGCGGAACTCACGGCTCGGTTTGGCTGCAATAAATGACACCACTAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCCCCCGCGACCAGaaa                                                                                                  >  2:491753/1‑139 (MQ=255)                                            tgttgtTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGa                                                                                           >  2:362086/1‑139 (MQ=255)                                                 tttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAg                                                                                            <  1:798763/133‑1 (MQ=255)                                                 tttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAg                                                                                            >  2:798763/1‑133 (MQ=255)                                                 tttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATAtt                                                                                      >  1:545226/1‑139 (MQ=255)                                                 tttgtGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATAtt                                                                                      >  2:52626/1‑139 (MQ=255)                                                   tgtgCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTgg                                                                                    >  2:416545/1‑139 (MQ=255)                                                           aGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTggg                                                                            <  2:73247/139‑1 (MQ=255)                                                               gcgcGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGa                                                                                                    >  2:181044/1‑111 (MQ=255)                                                               gcacgAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGa                                                                                                    <  1:181044/108‑1 (MQ=255)                                                                cgcgAACGGGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTATCACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGt                                                                       <  1:491753/139‑1 (MQ=255)                                                                  cgAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGa                                                                                                    <  1:612435/108‑1 (MQ=255)                                                                  cgAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGa                                                                                                    >  2:612435/1‑108 (MQ=255)                                                                      cgcgGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTcc                                                                 <  2:120783/139‑1 (MQ=255)                                                                                      cGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGt                                                 <  2:143717/139‑1 (MQ=255)                                                                                        cTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTcc                                               <  2:162882/139‑1 (MQ=255)                                                                                                 cAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 <  1:526476/48‑1 (MQ=255)                                                                                                 cAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACACCCCAGt                                                                                                                                 >  2:526476/1‑48 (MQ=255)                                                                                                           ccccACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCg                                                                                 <  2:30543/86‑1 (MQ=255)                                                                                                           ccccACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCg                                                                                 >  1:30543/1‑86 (MQ=255)                                                                                                           ccccACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGc                            <  1:362086/139‑1 (MQ=255)                                                                                                              cACGAAGATCCCTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAg                         >  2:138235/1‑139 (MQ=255)                                                                                                                         cTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAgcgc              >  2:650102/1‑139 (MQ=255)                                                                                                                         cTAACACCAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAgcgc              >  1:496195/1‑139 (MQ=255)                                                                                                                                cAGGAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGTTAAAAGCGCAATCaaa       <  2:292114/139‑1 (MQ=255)                                                                                                                                  ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCa                                                                                             <  2:397544/51‑1 (MQ=255)                                                                                                                                  ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCa                                                                                             >  1:397544/1‑51 (MQ=255)                                                                                                                                  ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTgg                                                                             <  2:491854/67‑1 (MQ=255)                                                                                                                                  ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTgg                                                                             >  1:491854/1‑67 (MQ=255)                                                                                                                                  ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTcc                                               <  1:47082/97‑1 (MQ=255)                                                                                                                                  ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTcc                                               >  2:47082/1‑97 (MQ=255)                                                                                                                                  ggAAAACACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAAcc     >  2:260925/1‑139 (MQ=255)                                                                                                                                     aaaCACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGAt  <  2:496195/139‑1 (MQ=255)                                                                                                                                     aaaCACCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGAt  <  1:260925/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       TGGCACCGAAGCCCATCAGTACACCACCTAACCCGCTACGTAATGTTGTTTGTGCATCAGCTGCGCGAACGCGGAACTCACGGCTCGCTTTGGCTGCAATAAATGACCCCACGAAGATCCCTAACACCAGGAAAACGCCCCAGTTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGAT  >  NC_000913/2084637‑2084907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑