Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	183,620	G→A	*386* (TAG→TAA)	cdaR →	carbohydrate diacid regulon transcriptional regulator, autoregulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	183,620	0	G	A	100.0%	102.1 / NA	34	*386* (TAG→TAA)	cdaR	carbohydrate diacid regulon transcriptional regulator, autoregulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/11);  total (23/11)

AGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGAT  >  NC_000913/183487‑183753                                                                                                                                      |                                                                                                                                      aGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATg                                                                                                                                 >  2:602414/1‑139 (MQ=255)      ttgttTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGTTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTa                                                                                                                            >  1:419201/1‑139 (MQ=255)                  cGTAATATCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggcgg                                                                                                                >  2:604362/1‑139 (MQ=255)                    tAATACCCTGGCGTAGCGGCTTAATCGTGTATCGGAACTGACCGGGCTTGATTTGGGAAATTTTGATGATAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑GGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                              <  1:437420/139‑1 (MQ=255)                          ccTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCt                                                                                                        >  1:202916/1‑139 (MQ=255)                                   tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                              <  1:146354/124‑1 (MQ=255)                                   tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                              >  2:146354/1‑124 (MQ=255)                                            tCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                      <  2:419201/139‑1 (MQ=255)                                                      taaCTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCAt                                                                                                     >  1:501595/2‑114 (MQ=255)                                                      gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTCAGGCGGCGCTGGCTCAt                                                                                                     <  2:501595/114‑1 (MQ=255)                                                           gACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATg                                                                       >  2:397864/1‑139 (MQ=255)                                                            aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                      >  1:330281/1‑139 (MQ=255)                                                            aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                      >  2:452913/1‑139 (MQ=255)                                                            aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                      >  2:224199/1‑139 (MQ=255)                                                                       tttGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGTGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAAcc                                                           <  1:621837/139‑1 (MQ=255)                                                                           ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCa                                                       >  1:559204/1‑139 (MQ=255)                                                                                ttttGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                              <  1:69477/79‑1 (MQ=255)                                                                                ttttGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                              >  2:69477/1‑79 (MQ=255)                                                                                         cAGGTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTAttt                                         <  1:397864/139‑1 (MQ=255)                                                                                            gTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGt                                                         >  1:615640/1‑120 (MQ=255)                                                                                            gTTGCTGCTGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGt                                                         <  2:615640/120‑1 (MQ=255)                                                                                               gctgctGTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGt                                   >  1:740566/1‑139 (MQ=255)                                                                                                    tgTATGTGGCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGtt                              >  2:722668/1‑139 (MQ=255)                                                                                                           ggCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAg                       >  1:257488/1‑139 (MQ=255)                                                                                                           ggCGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAg                       <  2:740566/139‑1 (MQ=255)                                                                                                             cGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGAt                     <  2:330281/139‑1 (MQ=255)                                                                                                             cGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGAt                     >  2:360791/1‑139 (MQ=255)                                                                                                             cGTTACAACTGG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGAt                     >  1:769396/1‑139 (MQ=255)                                                                                                                         gtGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAAtc          >  1:11947/2‑139 (MQ=255)                                                                                                                         gtGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAAtc          >  2:176643/2‑139 (MQ=255)                                                                                                                          tGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAAtct         >  2:341610/1‑139 (MQ=255)                                                                                                                                 cGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGAt  >  1:218322/1‑139 (MQ=255)                                                                                                                                 cGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGCTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGtttt                            <  2:389502/113‑1 (MQ=255)                                                                                                                                 cGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGCTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGtttt                            >  1:389502/1‑113 (MQ=255)                                                                                                                                      |                                                                                                                                      AGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGAT  >  NC_000913/183487‑183753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑