Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,099,237	G→A	A126V (GCG→GTG)	ugd ←	UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,099,237	0	G	A	100.0%	181.3 / NA	56	A126V (GCG→GTG)	ugd	UDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/28);  total (28/28)

CACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGA  >  NC_000913/2099130‑2099371                                                                                                            |                                                                                                                                       cACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgattt                                                                                                         >  2:365271/1‑139 (MQ=255) cACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgattt                                                                                                         >  2:346539/1‑139 (MQ=255)              aCGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAAcc                                                                                            >  2:657554/1‑139 (MQ=255)              aCGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAAcc                                                                                            <  1:223157/139‑1 (MQ=255)                    aGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATaa                                                                                      <  2:259460/139‑1 (MQ=255)                     ggATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAg                                                                                     >  1:195306/1‑139 (MQ=255)                     ggATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAg                                                                                     >  1:92331/1‑139 (MQ=255)                     ggATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAg                                                                                     >  1:659419/1‑139 (MQ=255)                       aTGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   <  1:346539/139‑1 (MQ=255)                       aTGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   <  1:657554/139‑1 (MQ=255)                        tGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCGGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                        <  2:626283/117‑1 (MQ=255)                        tGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCGGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                        >  1:626283/1‑117 (MQ=255)                         ggAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAtt                                                                                 >  1:161735/1‑139 (MQ=255)                         ggAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAtt                                                                                 <  1:365271/139‑1 (MQ=255)                                  cGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAAc                                                                        >  1:774372/1‑139 (MQ=255)                                  cGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAAc                                                                        >  1:495566/1‑139 (MQ=255)                                    tAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTa                                                                      <  2:659419/139‑1 (MQ=255)                                      aaGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACg                                                                    <  2:495566/139‑1 (MQ=255)                                         ggCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAt                                                                                  <  1:304306/122‑1 (MQ=255)                                         ggCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGAt                                                                                  >  2:304306/1‑122 (MQ=255)                                           cTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCttt                                                               >  2:704691/1‑139 (MQ=255)                                           cTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCttt                                                               >  1:75369/1‑139 (MQ=255)                                                 ccTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                         <  1:608564/139‑1 (MQ=255)                                                       ggAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTc                                                   <  1:569338/139‑1 (MQ=255)                                                               tCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   >  2:749307/1‑99 (MQ=255)                                                               tCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGa                                                                                   <  1:749307/99‑1 (MQ=255)                                                               tCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTgg                                           <  2:92331/139‑1 (MQ=255)                                                                    ggAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTa                                      <  2:75369/139‑1 (MQ=255)                                                                        aaaatattatattCTGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGa                                  >  2:354672/4‑139 (MQ=255)                                                                              atatTTTCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAaataat                            <  2:195306/139‑1 (MQ=255)                                                                                  tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                        >  2:688194/1‑59 (MQ=255)                                                                                  tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                        <  1:688194/59‑1 (MQ=255)                                                                                  tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTgg                                           <  2:344929/120‑1 (MQ=255)                                                                                  tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTgg                                           >  1:344929/1‑120 (MQ=255)                                                                                  tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGa                                          >  2:704653/1‑121 (MQ=255)                                                                                  tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGa                                          <  1:704653/121‑1 (MQ=255)                                                                                  tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGt                        >  1:645262/1‑139 (MQ=255)                                                                                  tttCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGt                        >  2:551574/1‑139 (MQ=255)                                                                                    tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTc                                                   <  1:708529/110‑1 (MQ=255)                                                                                    tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTc                                                   <  1:643371/110‑1 (MQ=255)                                                                                    tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTc                                                   >  2:708529/1‑110 (MQ=255)                                                                                    tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTc                                                   >  2:643371/1‑110 (MQ=255)                                                                                    tCAGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGttt                      >  1:152980/1‑139 (MQ=255)                                                                                      aGTGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTa                    >  2:421375/1‑139 (MQ=255)                                                                                        tGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                        <  2:635232/53‑1 (MQ=255)                                                                                        tGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGttgatttg                                                                                                        >  1:635232/1‑53 (MQ=255)                                                                                        tGCGATATTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAgg                  >  1:705226/1‑139 (MQ=255)                                                                                              atTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATa            <  1:421375/139‑1 (MQ=255)                                                                                              atTTCTTATGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATa            <  2:161735/139‑1 (MQ=255)                                                                                                      tGCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTg    <  1:658464/139‑1 (MQ=255)                                                                                                       gCATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTgg   >  2:569426/1‑139 (MQ=255)                                                                                                        cATCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGa  <  2:152980/139‑1 (MQ=255)                                                                                                         aTCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCAt                                                                                        >  1:190523/1‑52 (MQ=255)                                                                                                         aTCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCAt                                                                                        <  2:190523/52‑1 (MQ=255)                                                                                                         aTCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTAc                                                         <  1:439440/83‑1 (MQ=255)                                                                                                         aTCACTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGtttt                     <  1:433316/119‑1 (MQ=255)                                                                                                            |                                                                                                                                       CACCGATGACAATACGTGAAGGATGGAGATTATCGTAAAGGGCTTTACCCTCACGGAGAAATTCCGGGGAGAATATAATATTTTCAGTGCGATATTTCTTATGCATCGCTGCGGTAAAACCAACGGGAACCGTTGATTTGATGACCATAACCGCATAAGGATTTATCTCAACTACGTCTTTAATTACTGATTCTACACTGGATGTATTGAAATAATTAGTTTTAGGATCATAGTCGGTTGGA  >  NC_000913/2099130‑2099371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑