Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,104,494 C→T V6I (GTC→ATC) 
*197* (TAG→TAA
wbbK ←
wbbJ ←
lipopolysaccharide biosynthesis protein
putative lipopolysaccharide biosynthesis O‑acetyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,104,4940CT100.0% 80.4 / NA 26V6I (GTC→ATC) 
*197* (TAG→TAA
wbbK
wbbJ
lipopolysaccharide biosynthesis protein
putative lipopolysaccharide biosynthesis O‑acetyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/13);  total (13/13)

ACTTTCTTTTAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGACTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACTCCATTACC  >  NC_000913/2104360‑2104625
                                                                                                                                      |                                                                                                                                   
aCTTTCTTTTAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATg                                                                                                                                 <  1:491055/139‑1 (MQ=255)
         tAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTccc                                                                                                                        <  1:94377/139‑1 (MQ=255)
            ctctTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATa                                                                                                                     >  1:136986/1‑139 (MQ=255)
               tttAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAAtt                                                                                                                  >  2:9806/1‑139 (MQ=255)
                   gCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGt                                                                                                              <  2:551822/139‑1 (MQ=255)
                         tGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATg                                                                                                        >  1:680905/1‑139 (MQ=255)
                          ggACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGa                                                                                                       <  2:680905/139‑1 (MQ=255)
                            aCTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGAtt                                                                                                     >  2:533940/1‑139 (MQ=255)
                              tAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGt                                                                                                   <  1:533940/139‑1 (MQ=255)
                                 tGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTAtt                                                                                                >  2:714775/1‑139 (MQ=255)
                                                          ttattaGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTcc                                                                       <  2:136986/139‑1 (MQ=255)
                                                               aGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACAGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCaa                                                                  >  2:791016/1‑139 (MQ=255)
                                                                    ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGtct                                                                                                            >  1:123088/1‑92 (MQ=255)
                                                                    ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGtct                                                                                                            <  2:123088/92‑1 (MQ=255)
                                                                    ctgcCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACa                                                             >  1:378436/1‑139 (MQ=255)
                                                                                      aaaTGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGaa                                           <  2:378436/139‑1 (MQ=255)
                                                                                                   aCCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCAt                                                                                                         <  2:28644/64‑1 (MQ=255)
                                                                                                   aCCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCAt                                                                                                         >  1:28644/1‑64 (MQ=255)
                                                                                                   aCCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGAtttt                                                                                   >  1:771021/1‑86 (MQ=255)
                                                                                                   aCCGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGAtttt                                                                                   <  2:771021/86‑1 (MQ=255)
                                                                                                     cGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGaa                            >  1:346138/1‑139 (MQ=255)
                                                                                                     cGCCAGTGGTAAAATTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGaa                            >  1:408095/1‑139 (MQ=255)
                                                                                                               aaaaTTGACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGa                  >  1:389985/1‑139 (MQ=255)
                                                                                                                     gACCGCAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGa            <  2:74622/139‑1 (MQ=255)
                                                                                                                          cAGAAACAACGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACTCCa       <  2:346138/139‑1 (MQ=255)
                                                                                                                               acaacGATTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACTCCATTAcc  <  1:714775/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
ACTTTCTTTTAACTCTTTAGCAGAATGGACTAATGCGATAAAACTGACATTTTCTTTATTATTAGTTGCTGCCAAAAATTTTTTCAAAATGGTAAATGGACCGCCAGTGGTAAAATTGACCGCAGAAACAACGACTATGCTTTTTCCCATAATTTGGTCTCATGATTGTATTTCTTTATGATTTTTGCTGGTACTCCCGCAATGACAGTATTTTCGGGAATAGAACCTCTAACAACAGAATTGGCGCCGACTACGACTCCATTACC  >  NC_000913/2104360‑2104625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: