Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,263,478	T→C	V6V (GTA→GTG)	fruB ←	fused fructose‑specific PTS enzymes: IIA component/HPr component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,263,478	0	T	C	100.0%	104.5 / NA	30	V6V (GTA→GTG)	fruB	fused fructose‑specific PTS enzymes: IIA component/HPr component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/20);  total (10/20)

AGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAA  >  NC_000913/2263345‑2263596                                                                                                                                      |                                                                                                                       aGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGa                                                                                                                   >  1:172804/1‑139 (MQ=255)    tttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATaa                                                                                                                <  2:206935/139‑1 (MQ=255)     ttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCGGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAAc                                                                                                               <  2:773893/139‑1 (MQ=255)       gCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGTCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTg                                                                                                             <  1:693215/139‑1 (MQ=255)             cgcgcgCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACAt                                                                                                       <  1:714662/139‑1 (MQ=255)               cgcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAg                                                                                                     <  1:482966/139‑1 (MQ=255)                     aGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTctcc                                                                                               >  2:742929/1‑139 (MQ=255)                                      gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaa                                                                              <  2:684499/139‑1 (MQ=255)                                               tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAg                                                                     <  1:122808/139‑1 (MQ=255)                                                aCATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGc                                                                    <  2:786723/139‑1 (MQ=255)                                                aCATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGc                                                                    <  2:426035/139‑1 (MQ=255)                                                      ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTctcctc                                                                                             <  1:431408/108‑1 (MQ=255)                                                      ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTctcctc                                                                                             >  2:431408/1‑108 (MQ=255)                                                      ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTAt                                                              >  2:412867/1‑139 (MQ=255)                                                            tGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGaaa                                                        >  2:64596/1‑139 (MQ=255)                                                              cACCAGCGCCGCAGCGACCTTGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGACGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCGCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGaaaaa                                                      >  1:240238/1‑139 (MQ=255)                                                              cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGaaaaa                                                      >  2:640417/1‑139 (MQ=255)                                                                    cgccgcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGaa                                                                                   >  2:384902/1‑104 (MQ=255)                                                                    cgccgcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGaa                                                                                   <  1:384902/104‑1 (MQ=255)                                                                                cTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTa                                    <  1:64596/139‑1 (MQ=255)                                                                                cTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTa                                    <  1:315127/139‑1 (MQ=255)                                                                                     gAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                               <  1:412867/139‑1 (MQ=255)                                                                                      aaTCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTa                              <  1:211377/139‑1 (MQ=255)                                                                                                  tttGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATcg                  <  2:172804/139‑1 (MQ=255)                                                                                                    tGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATcgcg                <  2:263529/139‑1 (MQ=255)                                                                                                        tcCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGa            >  1:91962/1‑139 (MQ=255)                                                                                                           ggCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTtctc                                                                                                <  2:229311/52‑1 (MQ=255)                                                                                                           ggCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTtctc                                                                                                >  1:229311/1‑52 (MQ=255)                                                                                                                  tCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCaa  <  1:742929/139‑1 (MQ=255)                                                                                                                  tCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCaa  <  1:640417/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                       AGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAA  >  NC_000913/2263345‑2263596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑