Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,331	T→C	R647R (CGT→CGC)	ileS →	isoleucyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,331	0	T	C	100.0%	128.7 / NA	36	R647R (CGT→CGC)	ileS	isoleucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/23);  total (13/23)

TGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAG  >  NC_000913/24198‑24458                                                                                                                                      |                                                                                                                                tGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGcgctgc                                                                                                                            >  1:91440/1‑139 (MQ=255)   tCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCg                                                                                                                          <  1:66447/139‑1 (MQ=255)   tCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGACCCTGAAACGCGCTGCCg                                                                                                                          <  2:430209/139‑1 (MQ=255)         tCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCt                                                                                                                    <  2:542140/139‑1 (MQ=255)              cGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTAtcgt                                                                                                               >  1:342746/1‑139 (MQ=255)               ggCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTAtcgtc                                                                                                              <  1:325104/139‑1 (MQ=255)               ggCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTAtcgtc                                                                                                              <  2:342746/139‑1 (MQ=255)                 cAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATcgtcgt                                                                                                            <  2:720714/139‑1 (MQ=255)                          ttCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAac                                                                                                   >  1:175429/1‑139 (MQ=255)                            cgccgcAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAacac                                                                                                 <  2:572698/139‑1 (MQ=255)                              ccgcAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACcg                                                                                               >  2:190478/1‑139 (MQ=255)                               cgcAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACcgc                                                                                              >  1:365642/1‑139 (MQ=255)                                    gATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCt                                                                                         <  2:175429/139‑1 (MQ=255)                                    gATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCt                                                                                         <  2:432177/139‑1 (MQ=255)                                                       gcgcGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGaa                                                                      >  1:8505/1‑139 (MQ=255)                                                          cgGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACgg                                                                   >  2:713637/1‑139 (MQ=255)                                                                  cTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATc                                                           <  2:272950/139‑1 (MQ=255)                                                                      gTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGc                                                       <  1:190478/139‑1 (MQ=255)                                                                       tCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCa                                                      >  1:285282/1‑139 (MQ=255)                                                                           tgggtggCATCAACCGACTACACCGGTGAAATGGCCATTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAg                                                  >  2:113948/1‑139 (MQ=255)                                                                                  cATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATcgtcgt                                                                                                            >  2:68337/1‑74 (MQ=255)                                                                                  cATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATcgtcgt                                                                                                            <  1:68337/74‑1 (MQ=255)                                                                                  cATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGt                                           <  1:457155/139‑1 (MQ=255)                                                                                     cAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGaa                                        <  1:713637/139‑1 (MQ=255)                                                                                       aCCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAAc                                      <  2:534064/139‑1 (MQ=255)                                                                                                acCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTCCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAgaga                             >  1:118000/1‑139 (MQ=255)                                                                                                   ggTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGAtgg                          <  1:78278/139‑1 (MQ=255)                                                                                                   ggTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGAtgg                          <  2:285282/139‑1 (MQ=255)                                                                                                   ggTGAAATGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGAtgg                          <  2:713101/139‑1 (MQ=255)                                                                                                       aaaTGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAgaga                             >  1:586502/1‑132 (MQ=255)                                                                                                       aaaTGGCCGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAgaga                             <  2:586502/132‑1 (MQ=255)                                                                                                             ccGTTTCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGa                <  2:91440/139‑1 (MQ=255)                                                                                                                 ttCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGc            >  1:586996/1‑139 (MQ=255)                                                                                                                  tCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCt           <  2:365642/139‑1 (MQ=255)                                                                                                                  tCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCt           <  2:631671/139‑1 (MQ=255)                                                                                                                  tCTGACGAGATCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCt           <  2:8505/139‑1 (MQ=255)                                                                                                                  tCTGACGACATCCTGAACCGCACTGCCGATATCTATCGTCGTATCCGTAACACCGCGCGCTTCATGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCt           <  1:96702/139‑1 (MQ=255)                                                                                                                           aTCCTGAAACGCGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAg  >  1:210636/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                TGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAG  >  NC_000913/24198‑24458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑