Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,384,227	A→G	V499A (GTT→GCT)	yfbK ←	Von Willebrand factor domain putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,384,227	0	A	G	100.0%	150.0 / NA	42	V499A (GTT→GCT)	yfbK	Von Willebrand factor domain putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/18);  total (24/18)

ACTGTTTGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAACTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAACTTATCAATTGATGCTTTTTGCCCGTTC  >  NC_000913/2384093‑2384361                                                                                                                                       |                                                                                                                                       aCTGTTTGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCagctaa                                                                                                                                    <  2:455767/139‑1 (MQ=255)             gctgcCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGActttcttt                                                                                                                        >  2:67066/1‑139 (MQ=255)                   aggaggTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTg                                                                                                                  <  2:737177/139‑1 (MQ=255)                              ttCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTcc                                                                                                       >  2:489358/1‑139 (MQ=255)                                  ggTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCg                                                                                                   >  2:566954/1‑139 (MQ=255)                                   gTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGa                                                                                                  >  1:603429/1‑139 (MQ=255)                                      ttCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAAtt                                                                                               >  2:134325/1‑139 (MQ=255)                                       tCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAAttt                                                                                              <  1:673949/139‑1 (MQ=255)                                        cAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAAtttt                                                                                             >  1:231584/1‑139 (MQ=255)                                        cAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAAtttt                                                                                             >  1:464551/1‑139 (MQ=255)                                                cgTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAgg                                                                                     >  1:602936/1‑139 (MQ=255)                                                          gCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCttt                                                                           >  2:623911/1‑139 (MQ=255)                                                          gCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCttt                                                                           >  2:571031/1‑139 (MQ=255)                                                                          tgctgcGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTc                                                           >  1:296683/1‑139 (MQ=255)                                                                           gctgcGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCg                                                          <  1:67066/139‑1 (MQ=255)                                                                           gctgcGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCg                                                          <  2:695749/139‑1 (MQ=255)                                                                            ctgcGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCt                                                                                                                   <  2:22863/81‑1 (MQ=255)                                                                            ctgcGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCt                                                                                                                   >  1:22863/1‑81 (MQ=255)                                                                             tgcGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCt                                                        >  1:657355/1‑139 (MQ=255)                                                                             tgcGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCt                                                        <  1:566954/139‑1 (MQ=255)                                                                             tgcGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCt                                                        <  1:678269/139‑1 (MQ=255)                                                                             tgcGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCt                                                        <  2:602936/139‑1 (MQ=255)                                                                               cgcgAAAGCGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAg                                                                                 <  1:707894/112‑1 (MQ=255)                                                                               cgcgAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAg                                                                                 >  2:707894/1‑112 (MQ=255)                                                                                  gAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAAttt                                                   <  2:464551/139‑1 (MQ=255)                                                                                   aaaaCGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTg                                                  <  2:657355/139‑1 (MQ=255)                                                                                              cTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGc                                       <  1:571031/139‑1 (MQ=255)                                                                                                     gggCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGt                                <  1:191600/139‑1 (MQ=255)                                                                                                     gggCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGt                                <  2:603429/139‑1 (MQ=255)                                                                                                      ggCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCt                                                        <  2:305957/114‑1 (MQ=255)                                                                                                      ggCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCt                                                        >  1:305957/1‑114 (MQ=255)                                                                                                      ggCGCGTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCAAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTa                               >  1:201121/1‑139 (MQ=255)                                                                                                        cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATcc                                            >  2:621562/1‑124 (MQ=255)                                                                                                        cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATcc                                            <  1:621562/124‑1 (MQ=255)                                                                                                        cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAAc                             >  1:731976/1‑139 (MQ=255)                                                                                                        cgcgTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCAAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAAc                             >  2:499653/1‑139 (MQ=255)                                                                                                           gTTTATTGTTGGCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAACTTa                          <  1:489358/139‑1 (MQ=255)                                                                                                                     ggCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAACTTATCAATTGATg                >  2:23902/1‑139 (MQ=255)                                                                                                                     ggCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAACTTATCAATTGATg                >  1:742092/1‑139 (MQ=255)                                                                                                                     ggCCCCAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAACTTATCAATTGATg                >  2:616654/1‑139 (MQ=255)                                                                                                                          cAGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAACTTATCAATTGATGCtttt           >  2:100312/1‑139 (MQ=255)                                                                                                                           aGCGGGAATTCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAACTTATCAATTGATGCttttt          >  1:107237/1‑139 (MQ=255)                                                                                                                                   ttCAGCTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAACTTATCAATTGATGCTTTTTGCCCGTTc  <  1:510387/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       ACTGTTTGATCTGCTGCCAGGAGGTATTGTTCAGGTATTCAGAACCGCGTAACTTTTGCCCATATGCAGCTACTGCTGCGCGAAAACGCATATCTTCAGAGGGCGCGTTTATTGTTGGCCCCAGCGGGAATTCAACTAACTGACTTTCTTTTCCCTGCGGGTATTTCCAGCGAATTTTTAACCAGGCCAGTTCTTTCGTTTTGTCCGATTTCGCTAATTTGTTATCCGGGGCATAGCGTAACTTATCAATTGATGCTTTTTGCCCGTTC  >  NC_000913/2384093‑2384361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑