Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,534,031 T→C intergenic (+10/‑35) ptsH → / → ptsI phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)/PEP‑protein phosphotransferase of PTS system (enzyme I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,534,0310TC100.0% 69.5 / NA 20intergenic (+10/‑35)ptsH/ptsIphosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)/PEP‑protein phosphotransferase of PTS system (enzyme I)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/11);  total (9/11)

TTTAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGTTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATT  >  NC_000913/2533905‑2534143
                                                                                                                              |                                                                                                                
tttAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAAt                                                                                                      <  2:224193/139‑1 (MQ=255)
     aCTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTc                                                                                                 <  1:779382/139‑1 (MQ=255)
         cAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACaa                                                                                             <  1:241307/139‑1 (MQ=255)
          aGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTc                                                                                                 <  2:572962/134‑1 (MQ=255)
          aGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTc                                                                                                 >  1:572962/1‑134 (MQ=255)
                 gggCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTa                                                                                          >  1:454056/1‑134 (MQ=255)
                 gggCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTa                                                                                          <  2:454056/134‑1 (MQ=255)
                        aCTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTAt                                                                              >  1:485274/1‑139 (MQ=255)
                                         gACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAg                                                             <  1:120480/139‑1 (MQ=255)
                                               ctcCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTc                                                                                                 >  2:679094/1‑97 (MQ=255)
                                               ctcCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTc                                                                                                 <  1:679094/97‑1 (MQ=255)
                                               ctcCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATccc                                                       >  2:798188/1‑139 (MQ=255)
                                                            gAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAACCTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCttt                                          <  2:485274/139‑1 (MQ=255)
                                                                                      tCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGctct                              <  1:356528/125‑1 (MQ=255)
                                                                                      tCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGCAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGctct                              >  2:356528/1‑125 (MQ=255)
                                                                                        tGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGAc              <  1:798188/139‑1 (MQ=255)
                                                                                         ggTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACg             >  1:556406/1‑139 (MQ=255)
                                                                                              aaCTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAg                                                                                            <  2:20091/55‑1 (MQ=255)
                                                                                              aaCTGATGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAg                                                                                            >  1:20091/1‑55 (MQ=255)
                                                                                                    tGGCGGAACTCGAGTAATTTCCCGGGCTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCAtt  >  1:317360/1‑139 (MQ=255)
                                                                                                                              |                                                                                                                
TTTAAACTGCAGACTCTGGGCCTGACTCAAGGTACCGTTGTGACTATCTCCGCAGAAGGCGAAGACGAGCAGAAAGCGGTTGAACATCTGGTTAAACTGATGGCGGAACTCGAGTAATTTCCCGGGTTCTTTTAAAAATCAGTCACAAGTAAGGTAGGGTTATGATTTCAGGCATTTTAGCATCCCCGGGTATCGCTTTCGGTAAAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATT  >  NC_000913/2533905‑2534143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: