Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,637,295	C→T	R21H (CGT→CAT)	der ←	GTPase, multicopy suppressor of ftsJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,637,295	0	C	T	100.0%	123.8 / NA	40	R21H (CGT→CAT)	der	GTPase, multicopy suppressor of ftsJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/19);  total (21/19)

TCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTT  >  NC_000913/2637166‑2637415                                                                                                                                  |                                                                                                                         tctGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATa                                                                                                                 >  2:653458/1‑139 (MQ=255)         aTCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGAt                                                                                                         <  2:410738/139‑1 (MQ=255)                                aGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAg                                                                                            >  2:152243/1‑129 (MQ=255)                                aGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAg                                                                                            <  1:152243/129‑1 (MQ=255)                                 gATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACa                                                                                 >  1:749553/1‑139 (MQ=255)                                  aTAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACaa                                                                                >  2:359808/1‑139 (MQ=255)                                     aaCTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAgcg                                                                             >  2:338304/1‑139 (MQ=255)                                       cTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGTCCGACAAGcgcg                                                                           >  2:627718/1‑139 (MQ=255)                                       cTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGcgcg                                                                           >  2:794672/1‑139 (MQ=255)                                            ggCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACcac                                                                      >  2:112975/1‑139 (MQ=255)                                                          gCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTa                                                        >  1:266170/1‑139 (MQ=255)                                                              gACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGc                                                    <  1:46755/139‑1 (MQ=255)                                                                   tACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACcac                                                                      >  2:748008/1‑116 (MQ=255)                                                                   tACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACcac                                                                      <  1:748008/116‑1 (MQ=255)                                                                          ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAg                                                                               <  2:448427/100‑1 (MQ=255)                                                                          ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAg                                                                               >  1:448427/1‑100 (MQ=255)                                                                          ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCa                                                >  1:154142/1‑131 (MQ=255)                                                                          ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCa                                                <  2:154142/131‑1 (MQ=255)                                                                          ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTtata                                        >  2:8668/1‑139 (MQ=255)                                                                          ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTtata                                        >  2:151213/1‑139 (MQ=255)                                                                          ggTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTtata                                        <  1:311542/139‑1 (MQ=255)                                                                            tCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATaaa                                      <  1:794672/139‑1 (MQ=255)                                                                            tCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATaaa                                      <  2:266170/139‑1 (MQ=255)                                                                             cACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTAcc                                                               >  1:205448/1‑113 (MQ=255)                                                                             cACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTAcc                                                               <  2:205448/113‑1 (MQ=255)                                                                              aCGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAatc                                    >  2:461267/1‑139 (MQ=255)                                                                              aCGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAatc                                    >  2:437456/1‑139 (MQ=255)                                                                                   tCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAg                               <  1:338304/139‑1 (MQ=255)                                                                                    cAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGa                              <  1:151213/139‑1 (MQ=255)                                                                                         ccGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacg                         <  1:8668/139‑1 (MQ=255)                                                                                          cGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacgc                        <  1:348389/139‑1 (MQ=255)                                                                                            tgAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGATAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAGATCATCAGACAacgcac                      >  2:494883/2‑139 (MQ=255)                                                                                              aaaTCCGCAACCAGCGCATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAacgcacgc                    <  1:437456/139‑1 (MQ=255)                                                                                                            cgcATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTc             >  2:565380/1‑132 (MQ=255)                                                                                                            cgcATCTCGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTc             <  1:565380/132‑1 (MQ=255)                                                                                                                tctcGGGTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGGTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCgttgtt  <  1:112975/139‑1 (MQ=255)                                                                                                                      gTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTa                                                        >  2:417785/1‑79 (MQ=255)                                                                                                                      gTGCGAGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTa                                                        <  1:417785/79‑1 (MQ=255)                                                                                                                           aGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCa                                                              <  2:415450/68‑1 (MQ=255)                                                                                                                           aGTTAGATGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCa                                                              >  1:415450/1‑68 (MQ=255)                                                                                                                                  |                                                                                                                         TCTGTGCCATCAATCCCGCCGGTATCAATACAGATAAACTCACGGCCTTCAATTTCCGCACGACCGTACTTACGGTCACGAGTCAGACCCGGGAAATCCGCAACCAGCGCATCTCGGGTGCGAGTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTT  >  NC_000913/2637166‑2637415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑