| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,723,065 | G→A | A113A (GCG→GCA) | pssA → | phosphatidylserine synthase, CDP‑diacylglycerol‑serine O‑phosphatidyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,723,065 | 0 | G | A | 100.0% | 132.6 / NA | 42 | A113A (GCG→GCA) | pssA | phosphatidylserine synthase, CDP‑diacylglycerol‑serine O‑phosphatidyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/18); total (24/18) | |||||||||||
CGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGT > NC_000913/2722933‑2723199 | cgcgTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTACGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGaa > 2:208591/1‑139 (MQ=255) gTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCg > 1:205052/1‑139 (MQ=255) gCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAg > 2:442601/1‑139 (MQ=255) cTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGa > 2:70288/1‑139 (MQ=255) tAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGAt > 1:128709/1‑139 (MQ=255) aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGt > 1:195674/1‑139 (MQ=255) aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGt > 2:308927/1‑139 (MQ=255) aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGt > 2:185391/1‑139 (MQ=255) tCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTAt > 1:618600/1‑139 (MQ=255) aCTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt < 2:662686/139‑1 (MQ=255) aTGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaat < 1:751773/139‑1 (MQ=255) aTGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaat < 1:779233/139‑1 (MQ=255) tgCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaatcaa < 2:128709/139‑1 (MQ=255) gCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGGAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaatcaat > 1:546097/1‑139 (MQ=255) tGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCg > 2:203662/1‑139 (MQ=255) cTGGTCGACTGGCATCATGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGGAAATCCGGGCGTAGATGTTCCGGTTTATGACGTTCCAATCAATACTCGTg < 2:546097/139‑1 (MQ=255) aaCGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACtt > 2:192607/1‑139 (MQ=255) ggACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTc > 2:98631/1‑97 (MQ=255) ggACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTc < 1:98631/97‑1 (MQ=255) ggACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAg > 1:745524/1‑139 (MQ=255) ttGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTat > 2:20330/1‑139 (MQ=255) tGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTatc > 1:615271/1‑139 (MQ=255) cGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATcgacga < 1:208591/139‑1 (MQ=255) cGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATcgacga < 2:571997/139‑1 (MQ=255) gCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATa < 2:195674/139‑1 (MQ=255) aaCACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaatcaa < 1:499979/80‑1 (MQ=255) aaCACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaatcaa > 2:499979/1‑80 (MQ=255) cacTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGc > 1:631122/1‑90 (MQ=255) cacTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGc < 2:631122/90‑1 (MQ=255) tAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGc > 1:597059/1‑130 (MQ=255) tAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGc < 2:597059/130‑1 (MQ=255) tAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTtat < 1:185391/139‑1 (MQ=255) aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGc < 1:231437/85‑1 (MQ=255) aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGc > 2:231437/1‑85 (MQ=255) aCTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaatcaa > 1:37539/1‑67 (MQ=255) aCTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaatcaa < 2:37539/67‑1 (MQ=255) gTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGc < 1:192607/139‑1 (MQ=255) gCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGaa < 1:442601/139‑1 (MQ=255) ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAAc > 2:440481/1‑139 (MQ=255) tGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt < 2:788611/44‑1 (MQ=255) tGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt > 1:788611/1‑44 (MQ=255) tGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGt > 2:558991/1‑139 (MQ=255) | CGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGT > NC_000913/2722933‑2723199 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |