Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,724,448 C→T H107H (CAC→CAT
*433* (TAG→TAA
yfiM →
kgtP ←
putative lipoprotein
alpha‑ketoglutarate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,724,4480CT100.0% 106.9 / NA 34H107H (CAC→CAT
*433* (TAG→TAA
yfiM
kgtP
putative lipoprotein
alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/20);  total (14/20)

TGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACG  >  NC_000913/2724319‑2724574
                                                                                                                                 |                                                                                                                              
tGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc                                                                                                                       <  1:200297/139‑1 (MQ=255)
               gTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTg                                                                                                        <  1:689577/139‑1 (MQ=255)
                tCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGc                                                                                                       >  2:443139/1‑139 (MQ=255)
                    aaGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATg                                                                                                   <  1:306249/139‑1 (MQ=255)
                                        cccGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATGCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGaa                                                                               >  1:694346/1‑139 (MQ=255)
                                                 aGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGccacca                                                                      <  1:772829/139‑1 (MQ=255)
                                                   cGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACg                                                                    <  2:54992/139‑1 (MQ=255)
                                                      ctggagctggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGcc                                                                 <  2:395911/139‑1 (MQ=255)
                                                      ctggagctggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGcc                                                                 >  1:550940/1‑139 (MQ=255)
                                                             tggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAgg                                                          <  2:184239/139‑1 (MQ=255)
                                                              ggaAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGt                                                         <  2:160486/139‑1 (MQ=255)
                                                                aaGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTca                                                       <  2:586259/139‑1 (MQ=255)
                                                                                              aGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc                                                                                                                       <  1:384257/45‑1 (MQ=255)
                                                                                              aGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc                                                                                                                       >  2:384257/1‑45 (MQ=255)
                                                                                              aGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGccac                                                                        <  1:473239/92‑1 (MQ=255)
                                                                                              aGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGccac                                                                        >  2:473239/1‑92 (MQ=255)
                                                                                               gCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTAt                        >  2:252341/1‑139 (MQ=255)
                                                                                                    ggCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGAtt                   >  2:350843/1‑139 (MQ=255)
                                                                                                     gCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc                                                                                                                       <  2:236257/38‑1 (MQ=255)
                                                                                                     gCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc                                                                                                                       >  1:236257/1‑38 (MQ=255)
                                                                                                             gTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTg                                                                                                        >  1:42083/1‑45 (MQ=255)
                                                                                                             gTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTg                                                                                                        <  2:42083/45‑1 (MQ=255)
                                                                                                              tCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACaa         <  1:111988/139‑1 (MQ=255)
                                                                                                                tGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGc                                       <  2:356576/107‑1 (MQ=255)
                                                                                                                tGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGc                                       >  1:356576/1‑107 (MQ=255)
                                                                                                                tGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACg       <  2:550940/139‑1 (MQ=255)
                                                                                                                   cAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATg                                                                                                   <  1:156082/44‑1 (MQ=255)
                                                                                                                   cAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATg                                                                                                   >  2:156082/1‑44 (MQ=255)
                                                                                                                   cAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGc                                                                           >  1:777458/1‑68 (MQ=255)
                                                                                                                   cAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGc                                                                           <  2:777458/68‑1 (MQ=255)
                                                                                                                   cAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTa    >  1:716257/1‑139 (MQ=255)
                                                                                                                     aCTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACg  <  2:716257/139‑1 (MQ=255)
                                                                                                                         aCCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGa            >  2:336164/1‑125 (MQ=255)
                                                                                                                         aCCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGa            <  1:336164/125‑1 (MQ=255)
                                                                                                                                 |                                                                                                                              
TGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACG  >  NC_000913/2724319‑2724574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: