Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,905,532	T→C	intergenic (‑114/‑25)	queE ← / → yqcG	7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis/membrane stress resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,905,532	0	T	C	100.0%	98.7 / NA	30	intergenic (‑114/‑25)	queE/yqcG	7‑carboxy‑7‑deazaguanine synthase, queosine biosynthesis/membrane stress resistance protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/18);  total (12/18)

CTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAA  >  NC_000913/2905398‑2905648                                                                                                                                       |                                                                                                                     ctcGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaata                                                                                                                  >  1:257875/1‑139 (MQ=255) ctcGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaata                                                                                                                  >  1:761010/1‑139 (MQ=255)     ttAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTa                                                                                                              <  2:288682/139‑1 (MQ=255)         tCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAgg                                                                                                          <  2:761010/139‑1 (MQ=255)           gagTATTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCACGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACAGATCGTCAATAATTAAAGGGa                                                                                                        <  1:712106/137‑1 (MQ=255)             gTACTGCATAGCATTCTCTGTGAAGTAGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCCAACCATATCGTCAATAATTAAAGGGAGt                                                                                                      <  1:427402/139‑1 (MQ=255)               aCTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTaa                                                                                                    <  2:257875/139‑1 (MQ=255)                     tAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGtatt                                                                                              >  1:620627/1‑139 (MQ=255)                                    aGTGGATAATTGTTAGTTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCTAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAAt                                                                               >  1:537163/1‑139 (MQ=255)                                           aaTTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaa                                                                        <  1:120970/139‑1 (MQ=255)                                            aTTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGaaaa                                                                       <  1:105935/139‑1 (MQ=255)                                                ttAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGa                                                                   >  2:693281/1‑139 (MQ=255)                                                 tAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGAt                                                                  <  2:344521/139‑1 (MQ=255)                                                  aaTTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGAtt                                                                 >  1:27475/1‑139 (MQ=255)                                                      attGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAATATGCGAAACATATCGTCAATAATTAAAGGGGGTAACATATTATGCCAGAAGAAAATAAAGAAAATGGATTTAAt                                                             <  2:537163/139‑1 (MQ=255)                                                       ttGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaataa                                                                                                                 >  2:197738/1‑86 (MQ=255)                                                       ttGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTcaataa                                                                                                                 <  1:197738/86‑1 (MQ=255)                                                        tGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAAt                                                             <  1:599080/137‑1 (MQ=255)                                                        tGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGACTTAAt                                                             >  2:599080/1‑137 (MQ=255)                                                                       aacaaTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCAc                                            <  1:693281/139‑1 (MQ=255)                                                                         caaTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCa                                          >  1:86952/1‑139 (MQ=255)                                                                                 tCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATtata                                  >  1:404881/1‑139 (MQ=255)                                                                                           aTTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGt                                                                                                      <  2:137971/61‑1 (MQ=255)                                                                                           aTTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGt                                                                                                      >  1:137971/1‑61 (MQ=255)                                                                                               tGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGt                                                                                                      <  2:783095/57‑1 (MQ=255)                                                                                               tGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGt                                                                                                      >  2:64791/1‑57 (MQ=255)                                                                                               tGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGt                                                                                                      >  1:783095/1‑57 (MQ=255)                                                                                               tGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGt                                                                                                      <  1:64791/57‑1 (MQ=255)                                                                                                         aggTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGttttt          >  2:608306/1‑139 (MQ=255)                                                                                                           gTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGt                                                                                          <  2:363590/57‑1 (MQ=255)                                                                                                           gTTTAAAGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGt                                                                                          >  1:363590/1‑57 (MQ=255)                                                                                                                aaGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATa   <  2:126945/139‑1 (MQ=255)                                                                                                                 aGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATaa  <  2:164346/139‑1 (MQ=255)                                                                                                                 aGAAAAATGCGAAACATATCGTCAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATaa  <  1:301666/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                     CTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAA  >  NC_000913/2905398‑2905648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑