Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,983,288	C→T	*279* (TAG→TAA)	kduI ←	hexuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,983,288	0	C	T	100.0%	172.7 / NA	54	*279* (TAG→TAA)	kduI	hexuronate isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/28);  total (26/28)

CGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTG  >  NC_000913/2983160‑2983422                                                                                                                                 |                                                                                                                                       cGCTTGCGCCAGCCCCAACGCCATCCCCTGATCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAAt                                                                                                                              >  1:44555/1‑139 (MQ=255)   cTTGCGCCAGCCCCAACGCCATCCCCTGATCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCt                                                                                                                            >  1:740877/1‑139 (MQ=255)   cTTGCGCCAGCCCCAACGCCATCCCCTGATCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCt                                                                                                                            >  1:380968/1‑139 (MQ=255)       cgcCAGCCCCAACGCCATCCCCTGATCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTaa                                                                                                                        >  2:624747/1‑139 (MQ=255)             ccccAACGCCATCCCCTGATCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCca                                                                                                                  >  1:536563/1‑139 (MQ=255)              cccAACGCCATCCCCTGATCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcac                                                                                                                 <  1:274892/139‑1 (MQ=255)              cccAACGCCATCCCCTGATCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcac                                                                                                                 <  2:380968/139‑1 (MQ=255)               ccAACGCCATCCCCTGATCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                <  2:740877/139‑1 (MQ=255)               ccAACGCCATCCCCTGATCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                <  1:555300/139‑1 (MQ=255)                 aaCGCCATCCCCTGATCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATg                                                                                                              <  1:594393/139‑1 (MQ=255)                   cGCCATCCCCTGATCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGt                                                                                                            <  1:624747/139‑1 (MQ=255)                    gCCATCCCCTGATCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTc                                                                                                           <  2:536563/139‑1 (MQ=255)                         ccccTGATCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCatat                                                                                                      <  1:318273/139‑1 (MQ=255)                                   agtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                            <  1:324289/139‑1 (MQ=255)                                    gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                >  2:741304/1‑118 (MQ=255)                                    gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                <  1:741304/118‑1 (MQ=255)                                          tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                >  2:601027/1‑112 (MQ=255)                                          tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                <  1:601027/112‑1 (MQ=255)                                          tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGttt                                                                                     >  2:678542/1‑139 (MQ=255)                                          tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGttt                                                                                     >  1:425366/1‑139 (MQ=255)                                          tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGttt                                                                                     >  2:711412/1‑139 (MQ=255)                                               caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                >  2:777310/1‑107 (MQ=255)                                               caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                <  1:777310/107‑1 (MQ=255)                                               caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcac                                                                                                                 >  1:255188/1‑106 (MQ=255)                                               caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcac                                                                                                                 <  2:255188/106‑1 (MQ=255)                                               caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATCAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCAcc                                                                                >  2:428111/1‑139 (MQ=255)                                                   cAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                                            <  2:425366/139‑1 (MQ=255)                                                      tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCaa                                                                                                >  1:710519/1‑116 (MQ=255)                                                      tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCaa                                                                                                <  2:710519/116‑1 (MQ=255)                                                      tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATg                                                                         <  1:428111/139‑1 (MQ=255)                                                             cGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAc                                                                             >  1:392063/1‑128 (MQ=255)                                                             cGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAc                                                                             <  2:392063/128‑1 (MQ=255)                                                                       ccTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                >  2:656892/1‑83 (MQ=255)                                                                       ccTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                <  1:656892/83‑1 (MQ=255)                                                                                   aaTGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTcc                                            <  1:711412/139‑1 (MQ=255)                                                                                   aaTGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTcc                                            <  1:678542/139‑1 (MQ=255)                                                                                                    aaCAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                       >  1:492558/1‑95 (MQ=255)                                                                                                    aaCAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                       <  2:492558/95‑1 (MQ=255)                                                                                                     aCAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                          <  2:695550/139‑1 (MQ=255)                                                                                                         tACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAg                      >  1:131726/1‑139 (MQ=255)                                                                                                         tACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAg                      >  1:235329/1‑139 (MQ=255)                                                                                                             ttATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCg                  <  2:235329/139‑1 (MQ=255)                                                                                                              tATTCGTTTATGCCCACAATTAGCGCAAATCTTTaa                                                                                                                        >  1:753999/1‑36 (MQ=255)                                                                                                              tATTCGTTTATGCCCACAATTAGCGCAAATCTTTaa                                                                                                                        <  2:753999/36‑1 (MQ=255)                                                                                                               aTTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACGTGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCggg                <  2:622738/139‑1 (MQ=255)                                                                                                                ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg               >  1:95925/1‑139 (MQ=255)                                                                                                                 tCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                            >  2:642219/1‑61 (MQ=255)                                                                                                                 tCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                            <  1:642219/61‑1 (MQ=255)                                                                                                                 tCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGga              >  2:128424/1‑139 (MQ=255)                                                                                                                   gTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                                            <  2:115014/75‑1 (MQ=255)                                                                                                                   gTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                                            >  1:115014/1‑75 (MQ=255)                                                                                                                      tATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCa         >  1:273748/1‑139 (MQ=255)                                                                                                                          cccACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGc     >  1:772853/1‑139 (MQ=255)                                                                                                                             acaATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTg  <  2:586554/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       CGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTG  >  NC_000913/2983160‑2983422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑