Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,015,738	G→A	*193* (TAG→TAA)	mocA →	CTP:molybdopterin cytidylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,015,738	0	G	A	100.0%	102.6 / NA	33	*193* (TAG→TAA)	mocA	CTP:molybdopterin cytidylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/17);  total (16/17)

AATGTCACCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTC  >  NC_000913/3015604‑3015863                                                                                                                                       |                                                                                                                              aaTGTCACCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTaaaaaa                                                                                                                           <  1:69692/139‑1 (MQ=255)  aTGTCACCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAAc                                                                                                                          <  1:778063/139‑1 (MQ=255)   tGTCACCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACg                                                                                                                         <  1:533706/139‑1 (MQ=255)        ccAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGtt                                                                                                                    >  2:427154/1‑139 (MQ=255)              tGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGa                                                                                                              <  1:190363/139‑1 (MQ=255)                     ggCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGtt                                                                                                       >  2:19240/1‑139 (MQ=255)                                      aTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTc                                                                                      <  2:694539/139‑1 (MQ=255)                                      aTCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTc                                                                                      >  2:278899/1‑139 (MQ=255)                                          ttattCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGc                                                                                  <  2:398702/139‑1 (MQ=255)                                           tattCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                 <  2:123868/139‑1 (MQ=255)                                           tattCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                 <  1:755397/139‑1 (MQ=255)                                                cGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCag                                                                            <  1:427154/139‑2 (MQ=255)                                                  tCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTa                                                                          >  2:519577/1‑139 (MQ=255)                                                     aaaTAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCa                                                                       <  1:278899/139‑1 (MQ=255)                                                         aGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAAt                                                                   <  1:519577/139‑1 (MQ=255)                                                                                         cTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTgagaaaagagaa                                   >  1:27213/1‑139 (MQ=255)                                                                                              gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAAtt                              >  2:607955/1‑139 (MQ=255)                                                                                              gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAAtt                              >  2:749778/1‑139 (MQ=255)                                                                                              gacgacTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAAtt                              >  1:43230/1‑139 (MQ=255)                                                                                                      tattaCAGCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATg                      >  2:411806/1‑139 (MQ=255)                                                                                                             gCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGCAAAATTGATAAATGGGCTAGc               >  1:471415/1‑139 (MQ=255)                                                                                                             gCGAAGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGc               <  2:43230/139‑1 (MQ=255)                                                                                                                aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                               >  1:700752/1‑72 (MQ=255)                                                                                                                aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACt                                                                               <  2:700752/72‑1 (MQ=255)                                                                                                                aaGGAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCaat            >  1:366179/1‑139 (MQ=255)                                                                                                                  ggAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCaataa          <  2:366179/139‑1 (MQ=255)                                                                                                                  ggAAAGGTATACTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCaataa          <  1:19240/139‑1 (MQ=255)                                                                                                                          ataCTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTc  >  1:670920/1‑139 (MQ=255)                                                                                                                           taCTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTg                                                    >  1:727195/1‑88 (MQ=255)                                                                                                                           taCTGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTg                                                    <  2:727195/88‑1 (MQ=255)                                                                                                                              tGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                 <  1:48094/56‑1 (MQ=255)                                                                                                                              tGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCa                                                                                 >  2:48094/1‑56 (MQ=255)                                                                                                                              tGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATa                                                                     <  2:348995/68‑1 (MQ=255)                                                                                                                              tGAAATTTAAAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATa                                                                     >  1:348995/1‑68 (MQ=255)                                                                                                                                       |                                                                                                                              AATGTCACCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGTATACTGAAATTTAGAAAACGTAGTTAAACGATTGCGTTCAAATATTTAATCCTTCCGGCACTCATTATCATAATGATAAGGAATCACTGATTTTGAGAAAAGAGAAAAATTGATAAATGGGCTGGCAATAATAATTTTC  >  NC_000913/3015604‑3015863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑