Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,035,546	T→C	T246A (ACG→GCG)	prfB ←	peptide chain release factor RF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,035,546	0	T	C	100.0%	111.5 / NA	32	T246A (ACG→GCG)	prfB	peptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/10);  total (22/10)

GGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCA  >  NC_000913/3035450‑3035677                                                                                                 |                                                                                                                                    ggTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                                                           >  2:376628/1‑139 (MQ=255)   tCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTc                                                                                              >  2:473914/1‑134 (MQ=255)   tCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTc                                                                                              <  1:473914/134‑1 (MQ=255)                 ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGAttt                                                                                               >  2:408883/1‑119 (MQ=255)                 ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGAttt                                                                                               <  1:408883/119‑1 (MQ=255)                 ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATAtcatcatca                                                                           >  2:366612/1‑139 (MQ=255)                 ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATAtcatcatca                                                                           >  1:226580/1‑139 (MQ=255)                 ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATAtcatcatca                                                                           >  2:40367/1‑139 (MQ=255)                                    aTGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAAcg                                                        <  1:376628/139‑1 (MQ=255)                                      gtgGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAAcgcg                                                      <  1:420033/139‑1 (MQ=255)                                                cGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAacg                                            <  2:102565/139‑1 (MQ=255)                                                   aCCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCa                                                                                                                        >  2:2243/1‑60 (MQ=255)                                                   aCCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCa                                                                                                                        <  1:2243/60‑1 (MQ=255)                                                   aCCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAacgacg                                         >  1:267149/1‑139 (MQ=255)                                                       cAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGtgtg                                     >  1:484281/1‑139 (MQ=255)                                                        aGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTgg                                    >  1:325866/1‑139 (MQ=255)                                                        aGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTgg                                    >  1:101134/1‑139 (MQ=255)                                                             cGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGcgacg                               >  2:50492/1‑139 (MQ=255)                                                             cGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGcgacg                               <  2:226580/139‑1 (MQ=255)                                                               gtgcGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATgcg                                                                                                                   <  2:687787/50‑1 (MQ=39)                                                               gTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATgcg                                                                                                                   >  1:687787/1‑53 (MQ=255)                                                               gTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCgacgac                             >  1:704121/1‑139 (MQ=255)                                                                     ttAACGTGCTGACCGCCCGCGCCGGACGCGCGCTAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaatatc                                                                                  <  2:512558/80‑1 (MQ=255)                                                                     ttAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaatatc                                                                                  >  1:512558/1‑80 (MQ=255)                                                                             cTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAAc               >  2:175483/1‑139 (MQ=255)                                                                             cTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAAc               >  2:761353/1‑139 (MQ=255)                                                                               gACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACgg             >  1:11663/1‑139 (MQ=255)                                                                                    cccgcGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATGTCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCttt        >  1:345772/1‑139 (MQ=255)                                                                                     ccgcGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCtttt       >  1:554869/1‑139 (MQ=255)                                                                                      cgcgCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTg                                                                                                   <  1:102631/46‑1 (MQ=255)                                                                                      cgcgCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTg                                                                                                   >  2:102631/1‑46 (MQ=255)                                                                                          ccGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCa  >  2:781969/1‑139 (MQ=255)                                                                                                 |                                                                                                                                    GGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCA  >  NC_000913/3035450‑3035677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑