Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	280,024	C→T	pseudogene (94/174 nt)	insI1 →	IS30 transposase,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	280,024	0	C	T	100.0%	38.0 / NA	14	pseudogene (94/174 nt)	insI1	IS30 transposase,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/1);  total (13/1)

AATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAA  >  NC_000913/279907‑280145                                                                                                                      |                                                                                                                          aaTCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGa                                                                                                      >  1:246796/1‑139 (MQ=255)  aTCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGac                                                                                                     >  1:370017/1‑139 (MQ=255)                       ccGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGaa                                                                                <  2:463004/139‑1 (MQ=14)                                                                             cccAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCttttt                          >  1:421574/1‑139 (MQ=21)                                                                             cccAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCttttt                          >  2:700576/1‑139 (MQ=25)                                                                                  tataCTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAat                     >  1:321688/1‑139 (MQ=255)                                                                                  tataCTCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAat                     >  2:451513/1‑139 (MQ=255)                                                                                       tCAACATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTc                >  1:337707/1‑139 (MQ=255)                                                                                           cATGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGt            >  1:776026/1‑139 (MQ=255)                                                                                            aTGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt           >  1:129964/1‑139 (MQ=255)                                                                                            aTGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt           >  1:526691/1‑139 (MQ=255)                                                                                            aTGAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGtt           >  2:99370/1‑139 (MQ=255)                                                                                              gAACTAGATCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTtcg         >  1:23500/1‑139 (MQ=255)                                                                                                     aTCTGGTTGCTGCTCAGTTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTaa  >  1:764119/1‑139 (MQ=255)                                                                                                                      |                                                                                                                          AATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAGCAGCTTTTTTTAATATGTCCCGTTCGTCGGTAA  >  NC_000913/279907‑280145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑