Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,056,273	G→A	P11P (CCG→CCA)	fau →	5‑formyltetrahydrofolate cyclo‑ligase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,056,273	0	G	A	100.0%	146.7 / NA	46	P11P (CCG→CCA)	fau	5‑formyltetrahydrofolate cyclo‑ligase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/26);  total (20/26)

ACAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAAC  >  NC_000913/3056138‑3056403                                                                                                                                         |                                                                                                                                   aCAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaa                                                                                                                                 >  1:66719/1‑139 (MQ=255)      cTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAacaaca                                                                                                                            >  2:783356/1‑139 (MQ=255)         cggcggCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAAcagca                                                                                                                         >  2:605301/1‑139 (MQ=255)                        gaTTCCCTTCGTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTcaaca                                                                                                          <  2:69876/139‑1 (MQ=255)                            cccTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGcc                                                                                                      >  2:508806/1‑139 (MQ=255)                             ccTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGaaa                                                                                                                    <  2:414581/124‑1 (MQ=255)                             ccTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGaaa                                                                                                                    >  1:414581/1‑124 (MQ=255)                             ccTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCg                                                                                                     >  1:357572/1‑139 (MQ=255)                             ccTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCg                                                                                                     <  1:198831/139‑1 (MQ=255)                                 cttATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTAc                                                                                                 >  1:681400/1‑139 (MQ=255)                                    aTCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCg                                                                                              >  2:235106/1‑139 (MQ=255)                                    aTCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCg                                                                                              >  2:172821/1‑139 (MQ=255)                                       tGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgat                                                                                           >  1:345423/1‑139 (MQ=255)                                        ggCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatg                                                                                          >  2:379776/1‑139 (MQ=255)                                        ggCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatg                                                                                          <  1:172821/139‑1 (MQ=255)                                          cACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatgat                                                                                        <  1:508806/139‑1 (MQ=255)                                              aGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACt                                                                                    <  1:25212/139‑1 (MQ=255)                                                     aCGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATcccc                                                                             <  2:458359/139‑1 (MQ=255)                                                            tACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCggtggt                                                                      <  1:613503/139‑1 (MQ=255)                                                            tACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCggtggt                                                                      <  2:357572/139‑1 (MQ=255)                                                              ccAGAACTCCCACTGACATTATCCCGACCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                                     >  2:17034/1‑139 (MQ=255)                                                                aGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATg                                                                  <  1:235106/139‑1 (MQ=255)                                                                       ccACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATa                                                           >  1:249275/1‑139 (MQ=255)                                                                        cACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgat                                                                                           >  1:69951/1‑106 (MQ=255)                                                                        cACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgat                                                                                           <  2:69951/106‑1 (MQ=255)                                                                           tGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGt                                                       <  1:38173/139‑1 (MQ=255)                                                                           tGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGt                                                       <  2:275169/139‑1 (MQ=255)                                                                               aTTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                                     <  2:610543/121‑1 (MQ=255)                                                                               aTTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGgtggtg                                                                     >  1:610543/1‑121 (MQ=255)                                                                               aTTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCt                                                   <  2:249275/139‑1 (MQ=255)                                                                                 tATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCg                                                     >  2:360084/1‑135 (MQ=255)                                                                                 tATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCg                                                     <  1:360084/135‑1 (MQ=255)                                                                                        a‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCctc                                          >  1:634044/1‑139 (MQ=255)                                                                                          cAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaa                                                                                                                                 >  2:66095/1‑51 (MQ=255)                                                                                          cAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaa                                                                                                                                 <  1:66095/51‑1 (MQ=255)                                                                                          cAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATa                                                           >  2:554472/1‑121 (MQ=255)                                                                                          cAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATa                                                           <  1:554472/121‑1 (MQ=255)                                                                                              aaaTCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCttt                                     >  2:46677/1‑139 (MQ=255)                                                                                                     cAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGc                              <  2:345423/139‑1 (MQ=255)                                                                                                      caaaTAATTCTGCAACGTCGTCGTGCGTTAACGCCAACACACCAGCAGGATATGGGGCAACAGGCTGCTACCTGGGTGATGACTTATCCCCCGGGGGTGATGGCACATACGGTCGCTGTAGTCCTCTCTTTGGATGGCg                             <  1:13758/138‑1 (MQ=255)                                                                                                                   aaCGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCa                <  1:17034/139‑1 (MQ=255)                                                                                                                     cgtcgtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGc              <  2:66719/139‑1 (MQ=255)                                                                                                                     cgtcgtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGc              <  1:783356/139‑1 (MQ=255)                                                                                                                     cgtcgtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGc              <  1:714507/139‑1 (MQ=255)                                                                                                                      gtcgtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGcc             <  1:605301/139‑1 (MQ=255)                                                                                                                                 ttAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGaac  <  2:715608/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   ACAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGA‑CAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAAC  >  NC_000913/3056138‑3056403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑