Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,078,852	T→C	N3S (AAC→AGC)	cmtA ←	putative mannitol‑specific PTS IIB and IIC components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,078,852	0	T	C	100.0%	198.5 / NA	57	N3S (AAC→AGC)	cmtA	putative mannitol‑specific PTS IIB and IIC components
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/30);  total (27/30)

TCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGC  >  NC_000913/3078720‑3078982                                                                                                                                     |                                                                                                                                   tCGTTAGGCAGCCAACCGGTGGGAATACATAACGCAGTAATAACACCCCACGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCa                                                                                                                              <  2:594568/139‑1 (MQ=255) tCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGTCAATAAAAGCACCCATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCTAAAGCCTGGACCTTTGCACGATCAGACTTGCTTTCCa                                                                                                                              <  1:536362/139‑1 (MQ=255)      aGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCataata                                                                                                                         <  2:295348/139‑1 (MQ=255)        gCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAAtatt                                                                                                                       >  1:29053/1‑139 (MQ=255)         cAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCa                                                                                                                              >  2:331091/1‑131 (MQ=255)         cAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCa                                                                                                                              >  2:160191/1‑131 (MQ=255)         cAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCa                                                                                                                              <  1:331091/131‑1 (MQ=255)         cAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAAtatta                                                                                                                      <  2:306425/139‑1 (MQ=255)         cAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATCGCATTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCa                                                                                                                              <  1:160191/131‑1 (MQ=255)           gCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCCTTGCAGTCAAAAAGCCCTCAACGGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATc                                                                                                                    <  1:795869/139‑1 (MQ=255)           gCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATc                                                                                                                    <  1:194410/139‑1 (MQ=255)               tataagagacagaaataaCGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGTATGACCATTGCAGTCAAAAAGACCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGt                                                                                                                <  1:797637/127‑1 (MQ=255)                     gggAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAgagga                                                                                                          <  2:187515/139‑1 (MQ=255)                      ggAATAAATAATGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAgaggag                                                                                                         >  1:302339/1‑139 (MQ=255)                        aataaataACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGgagag                                                                                                       <  2:533162/139‑1 (MQ=255)                           aaataaCGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGt                                                                                                    >  1:376466/1‑139 (MQ=255)                                 cGCAGTAATAAAACCCCAGGCAATAAAAGCAACAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGCCTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTACTTAt                                                                                              >  2:570642/1‑139 (MQ=255)                                  gCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATc                                                                                             >  1:730868/1‑139 (MQ=255)                                  gCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATc                                                                                             >  1:126713/1‑139 (MQ=255)                                      taataaAACCCCAGGCAATATAATCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCACCCAAAAGCCTGGACCTTTGCACGATCAGACTTGCTTTCCTTAATATTATCCTGTAGAGGagagagctgtctcttata                                                                                         >  2:797637/1‑127 (MQ=255)                                      taataaAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcgcg                                                                                         >  1:455747/1‑139 (MQ=255)                                            aaCCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCTAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTAGCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGAt                                                                                   <  1:407417/139‑1 (MQ=255)                                              ccccAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCataa                                                                                                                           <  2:3501/97‑1 (MQ=255)                                              ccccAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCataa                                                                                                                           >  1:3501/1‑97 (MQ=255)                                                ccAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCataata                                                                                                                         <  1:588445/97‑1 (MQ=255)                                                ccAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCataata                                                                                                                         >  2:588445/1‑97 (MQ=255)                                                ccAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAgagga                                                                                                          >  1:611637/1‑112 (MQ=255)                                                ccAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAgagga                                                                                                          <  1:781573/112‑1 (MQ=255)                                                ccAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAgagga                                                                                                          <  2:611637/112‑1 (MQ=255)                                                ccAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAgagga                                                                                                          >  2:781573/1‑112 (MQ=255)                                                ccAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATg                                                                               >  2:781953/1‑139 (MQ=255)                                                 cAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGt                                                                              >  2:111667/1‑139 (MQ=255)                                                  aGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTc                                                                             >  2:239825/1‑139 (MQ=255)                                                             gCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGtt                                                                  >  2:240566/1‑139 (MQ=255)                                                                ccAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATc                                                                                                                    >  2:488581/1‑86 (MQ=255)                                                                ccAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATc                                                                                                                    <  1:488581/86‑1 (MQ=255)                                                                   atatTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATcc                                                                                            >  1:361767/1‑107 (MQ=255)                                                                   atatTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATcc                                                                                            <  2:361767/107‑1 (MQ=255)                                                                    tatTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCtga                                                           <  2:455747/139‑1 (MQ=255)                                                                      ttGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCtgatg                                                         <  2:756902/139‑1 (MQ=255)                                                                         gggATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                      <  1:240566/139‑1 (MQ=255)                                                                              gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGctt                                                                                                                                  >  1:527427/1‑58 (MQ=255)                                                                              gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATc                                                                                                                    <  2:160935/72‑1 (MQ=255)                                                                              gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATc                                                                                                                    >  1:160935/1‑72 (MQ=255)                                                                               aCCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcg                                                                                           >  2:413342/1‑96 (MQ=255)                                                                               aCCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcg                                                                                           <  1:413342/96‑1 (MQ=255)                                                                                   ttGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAagag                                            <  1:108290/139‑1 (MQ=255)                                                                                         tCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAAtatta                                                                                                                      <  2:527217/59‑1 (MQ=255)                                                                                         tCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTc                                      >  1:746710/1‑139 (MQ=255)                                                                                         tCAAAAACCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAAtatta                                                                                                                      >  1:527217/1‑59 (MQ=255)                                                                                            aaaaGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAg                                   <  2:29053/139‑1 (MQ=255)                                                                                            aaaaGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAg                                   <  2:746710/139‑1 (MQ=255)                                                                                                            cTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGcac                   <  1:642172/139‑1 (MQ=255)                                                                                                               gACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGt                >  2:346933/1‑139 (MQ=255)                                                                                                                     tGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCa          >  2:772262/1‑139 (MQ=255)                                                                                                                        aCGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACtt       <  1:111667/139‑1 (MQ=255)                                                                                                                            gCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACg   >  2:500474/1‑139 (MQ=255)                                                                                                                             cAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGc  <  1:500474/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   TCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGC  >  NC_000913/3078720‑3078982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑