Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,094,234 T→C T283A (ACA→GCA)  yggR ← putative PilT family AAA+ ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,094,2340TC100.0% 65.8 / NA 19T283A (ACA→GCA) yggRputative PilT family AAA+ ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/7);  total (12/7)

CAAACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTT  >  NC_000913/3094103‑3094345
                                                                                                                                   |                                                                                                               
ccaaCGCCCTTCCCCCACCCCGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATc                                                                                                          <  1:709856/137‑1 (MQ=255)
         ttCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCa                                                                                                 >  1:284839/1‑139 (MQ=255)
                     gTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCCAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcg                                                                                     >  1:2585/1‑139 (MQ=255)
                                  tggtgAAACGTGATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAGCTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGtt                                                                        >  1:465109/4‑139 (MQ=255)
                                  tgctgAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGtt                                                                        >  2:103098/1‑139 (MQ=255)
                                      gAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATc                                                                    <  2:284839/139‑1 (MQ=255)
                                              atcatcCCCACCTGCTGCCCGGTTTGAATAGCATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCa                                                            <  1:403596/139‑1 (MQ=255)
                                                               cccGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACt                                           >  1:181161/1‑139 (MQ=255)
                                                                    tttGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCg                                      >  1:451979/1‑139 (MQ=255)
                                                                      tGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTa                                    <  2:637225/139‑1 (MQ=255)
                                                                              aTGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCt                            >  2:416739/1‑139 (MQ=255)
                                                                                gCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGc                          >  2:260311/1‑139 (MQ=255)
                                                                                        tggtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGAt                  <  2:2585/139‑1 (MQ=255)
                                                                                         ggtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGAtt                 >  1:665042/1‑139 (MQ=255)
                                                                                                        cgcgAATCAAATTCCCCACCGTGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTc                                                              >  1:606167/1‑79 (MQ=255)
                                                                                                        cgcgAATCAAATTCCCCACCGTGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTc                                                              <  2:606167/79‑1 (MQ=255)
                                                                                                        cgcgAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCttt  >  1:634392/1‑139 (MQ=255)
                                                                                                         gcgAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcctt                                                                              <  2:136442/62‑1 (MQ=255)
                                                                                                         gcgAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcctt                                                                              >  1:136442/1‑62 (MQ=255)
                                                                                                                                   |                                                                                                               
CAAACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTT  >  NC_000913/3094103‑3094345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: