Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,176,858	C→T	*141* (TAG→TAA)	yqiB ←	DUF1249 protein YqiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,176,858	0	C	T	100.0%	106.0 / NA	34	*141* (TAG→TAA)	yqiB	DUF1249 protein YqiB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/15);  total (19/15)

TTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATA  >  NC_000913/3176725‑3176977                                                                                                                                      |                                                                                                                        ttACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTtaata                                                                                                                    >  1:719789/1‑139 (MQ=255)     cccTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAAcc                                                                                                                >  2:594847/1‑139 (MQ=255)     cccTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAAcc                                                                                                                >  2:255415/1‑139 (MQ=255)         aaCAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGaa                                                                                                            >  1:646157/1‑139 (MQ=255)              ggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGATGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGcc                                                                                                       <  1:547804/139‑1 (MQ=255)              ggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGcc                                                                                                       >  2:547804/1‑139 (MQ=255)              ggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGcc                                                                                                       <  1:594847/139‑1 (MQ=255)                      gCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTcc                                                                                               <  1:144606/139‑1 (MQ=255)                        ttttGTGCAAACAGGTGAGTGTCGGTAATTTGAAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACGGGAATCGCCATCGCTCCAt                                                                                             >  1:477762/1‑139 (MQ=255)                             tgCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCAt                                                                                             <  1:80869/134‑1 (MQ=255)                             tgCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCAt                                                                                             >  2:80869/1‑134 (MQ=255)                                aaaCAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAGTAAACCGGAATCGCCATCGCTCCATGTGCTaaa                                                                                     <  2:157811/139‑1 (MQ=255)                                           tgtCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCaa                                                                          >  2:263957/1‑139 (MQ=255)                                              cGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCa                                                                       >  2:326471/1‑139 (MQ=255)                                                                  aCTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTcc                                                                                               <  1:514241/95‑1 (MQ=255)                                                                  aCTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTcc                                                                                               >  2:514241/1‑95 (MQ=255)                                                                        gCCTCACCAGCCAGAAGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTACATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAAATTGAt                                             >  1:535829/1‑139 (MQ=255)                                                                             aCCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCg                                                                                                         >  2:29480/1‑74 (MQ=255)                                                                             aCCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCg                                                                                                         <  1:29480/74‑1 (MQ=255)                                                                                  ccagAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTtcgtc                                   >  1:658437/1‑139 (MQ=255)                                                                                      agGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCt                                                                <  1:676669/106‑1 (MQ=255)                                                                                      agGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCt                                                                >  2:676669/1‑106 (MQ=255)                                                                                      agGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGt                               >  2:8046/1‑139 (MQ=255)                                                                                            ggTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGc                         <  1:255415/139‑1 (MQ=255)                                                                                             gTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCa                        >  2:370351/1‑139 (MQ=255)                                                                                                aaCAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACt                     >  1:26210/1‑139 (MQ=255)                                                                                                      cTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttat               <  1:326471/139‑1 (MQ=255)                                                                                                       tttCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatt              <  2:646157/139‑1 (MQ=255)                                                                                                         tCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg            <  2:26210/139‑1 (MQ=255)                                                                                                         tCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg            <  2:719789/139‑1 (MQ=255)                                                                                                          ccAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCTGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAgg           <  1:486759/139‑1 (MQ=255)                                                                                                                   gtCCTTAGGTTCCACGACGTTAATAAACCGGAATCGCCATCGCTCCACGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATATGATGCTTTTCGTCGCGTTGATACAACTTTTTATTAGAATAATCATa  >  1:234632/1‑139 (MQ=255)                                                                                                                    tCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatt              >  1:357876/1‑126 (MQ=255)                                                                                                                    tCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatt              <  2:357876/126‑1 (MQ=255)                                                                                                                                      |                                                                                                                        TTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATA  >  NC_000913/3176725‑3176977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑