Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,269,602	G→A	*396* (TAG→TAA)	yhaC →	pentapetide repeats‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,269,602	0	G	A	100.0%	114.0 / NA	37	*396* (TAG→TAA)	yhaC	pentapetide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/18);  total (19/18)

CGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACG  >  NC_000913/3269467‑3269736                                                                                                                                        |                                                                                                                                       cGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACt                                                                                                                                     >  1:303129/1‑139 (MQ=255)  gagaAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACtt                                                                                                                                    >  1:694887/1‑139 (MQ=255)        gACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAAc                                                                                                                              <  2:642887/139‑1 (MQ=255)        gACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAAc                                                                                                                              >  1:642887/1‑139 (MQ=255)                ttCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTATGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                      <  2:152643/139‑1 (MQ=255)                ttCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                      <  2:694887/139‑1 (MQ=255)                 tctcCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACaa                                                                                                                     <  1:208704/139‑1 (MQ=255)                      ggACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCt                                                                                                                <  2:303129/139‑1 (MQ=255)                      ggACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCt                                                                                                                <  2:60285/139‑1 (MQ=255)                      ggACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTCAATAACTTCTTACAAAGTCt                                                                                                                <  1:658900/139‑1 (MQ=255)                                     ggTCGTGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTa                                                                                                 <  1:24402/139‑1 (MQ=255)                                              atatAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                                        <  2:335176/139‑1 (MQ=255)                                                       atatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                      <  1:41682/100‑1 (MQ=255)                                                       atatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                      >  2:41682/1‑100 (MQ=255)                                                       atatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAc                                                                               >  2:722541/1‑139 (MQ=255)                                                       atatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAc                                                                               >  1:770498/1‑139 (MQ=255)                                                        tatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                              <  1:176387/139‑1 (MQ=255)                                                               cATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                                        <  1:297680/122‑1 (MQ=255)                                                               cATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                                        >  2:297680/1‑122 (MQ=255)                                                                         agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCaa                                                             >  2:436651/1‑139 (MQ=255)                                                                           agaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCaaaa                                                           >  1:768218/1‑139 (MQ=255)                                                                           agaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCaaaa                                                           >  2:121697/1‑139 (MQ=255)                                                                                cAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                                        <  2:519732/105‑1 (MQ=255)                                                                                cAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                                        >  1:519732/1‑105 (MQ=255)                                                                                cAATATTTTACCACAGAGGACTATACGTATGAATACGACATTTTAAATGCAATATAACCTTAATAACTTCTTACAAAGTCTATTATTAATACCATAGATATTTATTCATCTTACCCTGTGCAACAAAAGCAATAACTCa                                                      >  1:692852/1‑139 (MQ=255)                                                                                              agagGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCATAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTg                                        >  2:256150/1‑139 (MQ=255)                                                                                                  gACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAAc                                    >  1:569860/1‑139 (MQ=255)                                                                                                        aCGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                              >  1:789065/1‑91 (MQ=255)                                                                                                        aCGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                              <  2:789065/91‑1 (MQ=255)                                                                                                            aTGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                      <  2:382008/47‑1 (MQ=255)                                                                                                            aTGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACa                                                                                                                      >  1:382008/1‑47 (MQ=255)                                                                                                               aTTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGt                                                                                                                  <  2:798189/48‑1 (MQ=255)                                                                                                               aTTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGt                                                                                                                  >  1:798189/1‑48 (MQ=255)                                                                                                                 tACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaa                     >  1:453864/1‑139 (MQ=255)                                                                                                                     aCCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAAt                 <  2:768218/139‑1 (MQ=255)                                                                                                                          ttAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTg            <  1:74376/139‑1 (MQ=255)                                                                                                                                    tataAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACg  >  2:49941/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       CGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACG  >  NC_000913/3269467‑3269736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑