Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,308,040	C→T	*295* (TAG→TAA)	nlpI ←	lipoprotein involved in osmotic sensitivity and filamentation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,308,040	0	C	T	100.0%	173.9 / NA	55	*295* (TAG→TAA)	nlpI	lipoprotein involved in osmotic sensitivity and filamentation
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/29);  total (26/29)

CTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCG  >  NC_000913/3307904‑3308169                                                                                                                                          |                                                                                                                                  cTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTa                                                                                                                                 >  2:32245/1‑139 (MQ=255)  tcatcaTGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTAt                                                                                                                                >  2:639385/1‑139 (MQ=255)  tcatcaTGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTAt                                                                                                                                >  1:644568/1‑139 (MQ=255)    atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTg                                                                                                                              >  1:451726/1‑139 (MQ=255)    atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTg                                                                                                                              >  1:701334/1‑139 (MQ=255)     tcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGc                                                                                                                             <  1:349757/139‑1 (MQ=255)              tGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGAt                                                                                                                    <  2:470957/139‑1 (MQ=255)                aTCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGAGTc                                                                                                                  >  1:16218/1‑139 (MQ=255)                            cATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATc                                                                                                      <  2:644568/139‑1 (MQ=255)                             aTTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                                     <  1:639385/139‑1 (MQ=255)                              ttGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCtt                                                                                                    <  2:451726/139‑1 (MQ=255)                              ttGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCtt                                                                                                    <  1:150290/139‑1 (MQ=255)                                   aaGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                               <  1:32245/139‑1 (MQ=255)                                      tgtgAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTgg                                                                                            >  2:313598/1‑139 (MQ=255)                                                cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCa                                                                                                             >  1:772379/1‑112 (MQ=255)                                                cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCa                                                                                                             <  2:772379/112‑1 (MQ=255)                                                cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                               >  2:357743/1‑126 (MQ=255)                                                cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                               <  1:357743/126‑1 (MQ=255)                                                cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                  >  2:718959/1‑139 (MQ=255)                                                cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGGCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                  <  1:665784/139‑1 (MQ=255)                                                      gTAGGTGTCTTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAAtt                                                                            <  2:477904/139‑1 (MQ=255)                                                          gtgtATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCcaa                                                                        >  2:355728/1‑139 (MQ=255)                                                          gtgtATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCcaa                                                                        >  1:85975/1‑139 (MQ=255)                                                            gtATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCcaaca                                                                      <  1:355728/139‑1 (MQ=255)                                                                    gAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTAt                                                              >  1:124238/1‑139 (MQ=255)                                                                    gAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTAt                                                              <  1:236316/139‑1 (MQ=255)                                                                    gAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTAt                                                              >  1:755456/1‑139 (MQ=255)                                                                     aacaacaacGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                               >  1:282954/1‑105 (MQ=255)                                                                     aacaacaacGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                               <  2:282954/105‑1 (MQ=255)                                                                       caacaacGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCATGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCgg                                                           >  1:27311/1‑139 (MQ=255)                                                                       caacaacGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                  <  2:644726/116‑1 (MQ=255)                                                                       caacaacGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                  >  1:644726/1‑116 (MQ=255)                                                                         acaacGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCATGTCATCTTGTTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTg                                                         <  1:495039/139‑1 (MQ=255)                                                                         acaacGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                                     >  2:54641/1‑95 (MQ=255)                                                                         acaacGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                                     <  1:54641/95‑1 (MQ=255)                                                                         acaacGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTg                                                         <  2:755456/139‑1 (MQ=255)                                                                         acaacGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTg                                                         <  2:701334/139‑1 (MQ=255)                                                                         acaacGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTg                                                         <  2:778064/139‑1 (MQ=255)                                                                               cccTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTcaac                                                   <  1:267696/139‑1 (MQ=255)                                                                               cccTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTcaac                                                   >  2:661437/1‑139 (MQ=255)                                                                                  tCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACaaa                                                >  1:70506/1‑139 (MQ=255)                                                                                                 aTGGCAATCAAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACgttgt                                  >  1:348167/1‑139 (MQ=255)                                                                                                  tGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                  >  2:306940/1‑89 (MQ=255)                                                                                                  tGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                  <  1:306940/89‑1 (MQ=255)                                                                                                   ggCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTgg                               >  2:476525/1‑139 (MQ=255)                                                                                                        tC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAAcc                          >  1:540047/1‑139 (MQ=255)                                                                                                         c‑AAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                         <  1:313598/139‑1 (MQ=255)                                                                                                            aaaaaGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGcc                       <  1:1474/139‑1 (MQ=255)                                                                                                               aaGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGt                    <  2:70506/139‑1 (MQ=255)                                                                                                                    tACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaa               <  2:61764/139‑1 (MQ=255)                                                                                                                    tACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaa               <  1:661437/139‑1 (MQ=255)                                                                                                                            gATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCg       >  1:159038/1‑139 (MQ=255)                                                                                                                             aTGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGcc                       <  2:566518/122‑1 (MQ=255)                                                                                                                             aTGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTGGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGcc                       >  1:566518/1‑122 (MQ=255)                                                                                                                                 gtACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCATCAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGgcg  <  1:493899/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  CTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATC‑AAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCG  >  NC_000913/3307904‑3308169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑