Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,414,727	G→A	P288P (CCG→CCA)	acrE →	cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,414,727	0	G	A	100.0%	89.5 / NA	29	P288P (CCG→CCA)	acrE	cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/13);  total (16/13)

CATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGC  >  NC_000913/3414601‑3414861                                                                                                                               |                                                                                                                                       cATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTtgtg                                                                                                                            <  1:134227/139‑1 (MQ=255)                                  tACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGAc                                                                                           >  2:364980/1‑139 (MQ=255)                                                            ttGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGt                                                                 <  1:414504/139‑1 (MQ=255)                                                            ttGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGt                                                                 <  2:36190/139‑1 (MQ=255)                                                                      cACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGcaca                                                       >  2:297297/1‑139 (MQ=255)                                                                       aCCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTc                                                                                                    >  2:541252/1‑93 (MQ=255)                                                                       aCCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTc                                                                                                    <  1:541252/93‑1 (MQ=255)                                                                         cGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACcg                                                    >  1:620659/1‑139 (MQ=255)                                                                            cTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCgt                                                 <  2:704695/139‑1 (MQ=255)                                                                                       tACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAAc                                      >  1:307254/1‑139 (MQ=255)                                                                                         cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGt                                                                                                                                 <  2:81122/46‑1 (MQ=255)                                                                                         cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGt                                                                                                                                 >  1:81122/1‑46 (MQ=255)                                                                                         cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGcaac                                                                        >  1:294078/1‑103 (MQ=255)                                                                                         cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGcaac                                                                        <  2:294078/103‑1 (MQ=255)                                                                                         cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGt                                                                 <  2:318759/110‑1 (MQ=255)                                                                                         cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGt                                                                 >  1:318759/1‑110 (MQ=255)                                                                                         cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCg                                    >  2:186083/1‑139 (MQ=255)                                                                                               gTCTTCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGa                              >  2:354223/1‑139 (MQ=255)                                                                                                   tCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGcaca                                                       <  1:474774/110‑1 (MQ=255)                                                                                                   tCCCTAACCCGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGcaca                                                       >  2:474774/1‑110 (MQ=255)                                                                                                          cccGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGca                                                         <  2:32301/101‑1 (MQ=255)                                                                                                          cccGCAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGca                                                         >  1:32301/1‑101 (MQ=255)                                                                                                              cAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCt                                                                                              <  1:741721/60‑1 (MQ=255)                                                                                                              cAACATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCt                                                                                              >  2:741721/1‑60 (MQ=255)                                                                                                               aaCATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAg                                                                                                         <  2:237112/48‑1 (MQ=255)                                                                                                               aaCATACGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAg                                                                                                         >  1:237112/1‑48 (MQ=255)                                                                                                                    aCGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGcaac                                                                        <  1:635698/76‑1 (MQ=255)                                                                                                                    aCGCTTTTGCCAGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGcaac                                                                        >  2:635698/1‑76 (MQ=255)                                                                                                                           tGCCAGGTATGTTTGTGCGTGCACGGAGTGATGAAGGCGTCCAACTTGACGCCCTCCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGGCATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGATTGAAgc  >  1:280897/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                       CATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGC  >  NC_000913/3414601‑3414861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑