Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,731,980 G→A G284S (GGT→AGT)  xylF → D‑xylose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,731,9800GA100.0% 167.0 / NA 52G284S (GGT→AGT) xylFD‑xylose transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/29);  total (23/29)

AGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATGGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCG  >  NC_000913/3731847‑3732115
                                                                                                                                     |                                                                                                                                       
aGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAg                                                                                                                                    <  2:409093/139‑1 (MQ=255)
aGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAg                                                                                                                                    <  1:452330/139‑1 (MQ=255)
   aaTCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGaa                                                                                                                                 >  1:638886/1‑139 (MQ=255)
      ctcCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCa                                                                                                                              <  1:593507/139‑1 (MQ=255)
         cGGCCAGGATGCGGATCTCGCAGGTATTTAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaaaa                                                                                                                           >  2:348328/1‑139 (MQ=255)
                  tGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATAcc                                                                                                                  <  2:638886/139‑1 (MQ=255)
                   gCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACca                                                                                                                 <  1:271526/139‑1 (MQ=255)
                    cGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACcac                                                                                                                >  1:773700/1‑139 (MQ=255)
                       aTCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACt                                                                                                             <  2:728971/139‑1 (MQ=255)
                       aTCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACt                                                                                                             <  2:329689/139‑1 (MQ=255)
                       aTCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACt                                                                                                             >  2:195387/1‑139 (MQ=255)
                          tcGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaa                                                                                                          >  2:662269/1‑139 (MQ=255)
                              aGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaataat                                                                                                      <  2:773700/139‑1 (MQ=255)
                              aGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaataat                                                                                                      <  1:609430/139‑1 (MQ=255)
                                      aaCGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGaa                                                                                              >  1:723560/1‑139 (MQ=255)
                                       aCGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGaaa                                                                                             <  1:348328/139‑1 (MQ=255)
                                          tATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGAt                                                                                          >  1:550691/1‑139 (MQ=255)
                                                  ccGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTc                                                                                  >  2:678932/1‑139 (MQ=255)
                                                     gTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                               <  1:195387/139‑1 (MQ=255)
                                                                       tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGa                                                                                                                      >  2:215435/1‑82 (MQ=255)
                                                                       tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGa                                                                                                                      <  1:215435/82‑1 (MQ=255)
                                                                       tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACcaca                                                                                                               <  2:248109/89‑1 (MQ=255)
                                                                       tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACcaca                                                                                                               >  1:248109/1‑89 (MQ=255)
                                                                       tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                               >  1:264729/1‑121 (MQ=255)
                                                                       tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                               <  2:264729/121‑1 (MQ=255)
                                                                              aCCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAAt                                                      <  1:213179/139‑1 (MQ=255)
                                                                              aCCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAAt                                                      >  2:292632/1‑139 (MQ=255)
                                                                                   ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACcgatcgat                                                            <  1:232821/128‑1 (MQ=255)
                                                                                   ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACcgatcgat                                                            >  2:232821/1‑128 (MQ=255)
                                                                                   ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaaaa                                                 >  1:660672/1‑139 (MQ=255)
                                                                                   ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaaaa                                                 <  1:662269/139‑1 (MQ=255)
                                                                                   ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaaaa                                                 >  1:213635/1‑139 (MQ=255)
                                                                                   ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaaaa                                                 >  1:291249/1‑139 (MQ=255)
                                                                                    tACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAaac                                                <  1:678932/139‑1 (MQ=255)
                                                                                    tACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAaac                                                <  2:213635/139‑1 (MQ=255)
                                                                                          gttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATc                                          <  1:586589/139‑1 (MQ=255)
                                                                                           ttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaataat                                                                                                      <  2:525512/78‑1 (MQ=255)
                                                                                           ttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaataat                                                                                                      >  1:525512/1‑78 (MQ=255)
                                                                                           ttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                               <  2:216328/101‑1 (MQ=255)
                                                                                           ttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                               >  1:216328/1‑101 (MQ=255)
                                                                                                        ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAAt                            >  2:16970/1‑139 (MQ=255)
                                                                                                            aGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTaaa                        >  1:340378/1‑139 (MQ=255)
                                                                                                             gAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACcgatc                                                               <  2:647240/99‑1 (MQ=255)
                                                                                                             gAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACcgatc                                                               >  1:647240/1‑99 (MQ=255)
                                                                                                               aaTTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGAc                     <  2:660672/139‑1 (MQ=255)
                                                                                                                     cGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACgg                   <  2:168926/135‑1 (MQ=255)
                                                                                                                     cGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACgg                   >  1:168926/1‑135 (MQ=255)
                                                                                                                               ggCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACcga                                                                 <  2:150373/79‑1 (MQ=255)
                                                                                                                               ggCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACcga                                                                 >  1:150373/1‑79 (MQ=255)
                                                                                                                                gCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAagagag    <  2:291249/139‑1 (MQ=255)
                                                                                                                                 cAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAgagc   <  1:16970/139‑1 (MQ=255)
                                                                                                                                  aaTAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAgagcg  <  2:15231/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
AGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATGGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCG  >  NC_000913/3731847‑3732115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑