Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,789,352	G→A	R244C (CGC→TGC)	waaH ←	LPS(HepIII)‑glucuronic acid glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,789,352	0	G	A	100.0%	166.3 / NA	52	R244C (CGC→TGC)	waaH	LPS(HepIII)‑glucuronic acid glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/32);  total (20/32)

CGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAG  >  NC_000913/3789216‑3789475                                                                                                                                         |                                                                                                                            cGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGcaggcagg                                                                                                                           >  2:564721/1‑139 (MQ=255) cGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGcaggcagg                                                                                                                           <  2:307619/139‑1 (MQ=255)  gTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                          <  2:25739/139‑1 (MQ=255)  gTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                          <  2:137046/139‑1 (MQ=255)  gTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                          <  1:515963/139‑1 (MQ=255)       cGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCt                                                                                                                     <  1:658676/139‑1 (MQ=255)       cGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCt                                                                                                                     <  1:564721/139‑1 (MQ=255)          gTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTaa                                                                                                                  <  1:415442/139‑1 (MQ=255)            aaGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAata                                                                                                                <  1:283734/139‑1 (MQ=255)             aGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAatat                                                                                                               >  2:485604/1‑139 (MQ=255)                atatCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGt                                                                                                            <  1:206176/139‑1 (MQ=255)                    ccGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCt                                                                                                                     <  2:580080/126‑1 (MQ=255)                    ccGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCt                                                                                                                     >  1:580080/1‑126 (MQ=255)                         tctTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGAt                                                                                                   >  2:55106/1‑139 (MQ=255)                                  aCCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGt                                                                                          <  2:719535/139‑1 (MQ=255)                                     gCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGtttt                                                                                       >  2:722923/1‑139 (MQ=255)                                       aTGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTa                                                                                     <  1:325348/139‑1 (MQ=255)                                           cAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTcc                                                                                 <  1:722923/139‑1 (MQ=255)                                           cAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTcc                                                                                 <  2:425397/139‑1 (MQ=255)                                                acGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGt                                                                            >  1:171414/1‑139 (MQ=255)                                                        ccTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCaa                                                                    <  1:55106/139‑1 (MQ=255)                                                           cGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTACGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCg                                                                 >  1:375275/1‑139 (MQ=255)                                                            gTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGa                                                                <  1:485604/139‑1 (MQ=255)                                                            gTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGa                                                                >  2:335900/1‑139 (MQ=255)                                                            gTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGa                                                                <  1:85638/139‑1 (MQ=255)                                                              aaGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACt                                                              >  1:632216/1‑139 (MQ=255)                                                                   aTTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTg                                                         <  2:110717/139‑1 (MQ=255)                                                                   aTTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTg                                                         >  1:742987/1‑139 (MQ=255)                                                                   aTTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTg                                                         <  2:632216/139‑1 (MQ=255)                                                                        cTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTaa                                                                                                                        <  1:388530/71‑1 (MQ=255)                                                                        cTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTaa                                                                                                                        >  2:388530/1‑71 (MQ=255)                                                                        cTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGta                                                    <  2:742987/139‑1 (MQ=255)                                                                           atgaAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAAt                                                                                                                       >  2:731434/1‑69 (MQ=255)                                                                           atgaAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAAt                                                                                                                       <  1:731434/69‑1 (MQ=255)                                                                           atgaAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCa                                                            <  1:61482/128‑1 (MQ=255)                                                                           atgaAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCa                                                            >  2:61482/1‑128 (MQ=255)                                                                             gaAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATg                                               <  1:497503/139‑1 (MQ=255)                                                                                  tCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGat                                          <  2:171414/139‑1 (MQ=255)                                                                                     ggATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                          <  2:39277/56‑1 (MQ=255)                                                                                     ggATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                          >  1:39277/1‑56 (MQ=255)                                                                                     ggATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGataat                                       >  1:40154/1‑139 (MQ=255)                                                                                      gATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGTAGGCAGGt                                                                                                                          <  2:376712/55‑1 (MQ=255)                                                                                      gATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGTAGGCAGGt                                                                                                                          >  1:376712/1‑55 (MQ=255)                                                                                      gATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGataata                                      >  2:770411/1‑139 (MQ=255)                                                                                                  ttttGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGa                          <  2:432705/139‑1 (MQ=255)                                                                                                     tGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                          >  2:802090/1‑40 (MQ=255)                                                                                                     tGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                                                                          <  1:802090/40‑1 (MQ=255)                                                                                                      gTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCt                                                                                                                     <  2:314955/44‑1 (MQ=255)                                                                                                      gTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCt                                                                                                                     >  1:314955/1‑44 (MQ=255)                                                                                                      gTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGc                      <  2:40154/139‑1 (MQ=255)                                                                                                      gTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGc                      >  2:27064/1‑139 (MQ=255)                                                                                                                         aaTTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGa                          >  2:424714/1‑116 (MQ=255)                                                                                                                         aaTTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGa                          <  1:424714/116‑1 (MQ=255)                                                                                                                          aTTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTAGGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAg  <  2:121673/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                            CGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAG  >  NC_000913/3789216‑3789475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑