Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,110,806 A→G G234G (GGT→GGC)  tpiA ← triosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,110,8060AG93.5% 95.4 / ‑2.1 31G234G (GGT→GGC) tpiAtriosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (13/16);  total (14/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

TGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGG  >  NC_000913/4110670‑4110938
                                                                                                                                        |                                                                                                                                    
tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGcgcc                                                                                                                                    >  1:409528/1‑139 (MQ=255)
          cGTTAAGGCGAAGCGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTAAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAgc                                                                                                                          >  1:339480/1‑139 (MQ=255)
                 gCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGt                                                                                                                   <  2:409528/139‑1 (MQ=255)
                                  gtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCa                                                                                                  >  2:389478/1‑139 (MQ=255)
                                     agtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAAc                                                                                               >  1:581211/1‑139 (MQ=255)
                                        gCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGt                                                                                            >  2:478852/1‑139 (MQ=255)
                                                      ccGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCAGCAGCGTTCGa                                                                              <  1:466110/139‑1 (MQ=255)
                                                                   gACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACagag                                                                 <  2:358109/139‑1 (MQ=255)
                                                                     cTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAgcc                                                               >  1:687933/1‑139 (MQ=255)
                                                                              gTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTg                                                      >  1:699599/1‑139 (MQ=255)
                                                                                ttAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGaa                                                    <  2:765435/139‑1 (MQ=255)
                                                                                    ccGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGt                                                                                                                   <  2:91246/72‑1 (MQ=255)
                                                                                    ccGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGt                                                                                                                   >  1:91246/1‑72 (MQ=255)
                                                                                            gCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGtt                                        <  2:692660/139‑1 (MQ=255)
                                                                                               gCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAg                                     <  1:426591/139‑1 (MQ=255)
                                                                                                    aaCGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGAcgcg                                                                          <  1:405784/97‑1 (MQ=255)
                                                                                                    aaCGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGAcgcg                                                                          >  2:405784/1‑97 (MQ=255)
                                                                                                     aCGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAg                                     <  1:687863/133‑1 (MQ=255)
                                                                                                     aCGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAg                                     >  2:687863/1‑133 (MQ=255)
                                                                                                          tACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCg                          <  2:335461/139‑1 (MQ=255)
                                                                                                          tACTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCg                          >  1:335461/1‑139 (MQ=255)
                                                                                                           aCTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGt                         <  2:581211/139‑1 (MQ=255)
                                                                                                            cTGCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTc                        <  2:159830/139‑1 (MQ=255)
                                                                                                              gCGAAGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCaa                      <  2:95258/139‑1 (MQ=255)
                                                                                                                 aaGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAgccg                                                              <  2:163169/96‑1 (MQ=255)
                                                                                                                 aaGGCGTCAGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAgccg                                                              >  1:163169/1‑96 (MQ=255)
                                                                                                                       tCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTAc                                                        <  2:600274/96‑1 (MQ=255)
                                                                                                                       tCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTAc                                                        >  1:600274/1‑96 (MQ=255)
                                                                                                                         aGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTa                                                                                >  2:45606/1‑70 (MQ=255)
                                                                                                                         aGCTTTCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTa                                                                                <  1:45606/70‑1 (MQ=255)
                                                                                                                              tCAGAGAAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTc      >  1:208847/1‑139 (MQ=255)
                                                                                                                                  agaAGCGCCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACgg  <  1:389478/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
TGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTTTACAGAGCCGCCGTACTGAATGATCACTTGTTCAGCGATGTTAGCGTCAACTTTAGCGATGTGGTCACGG  >  NC_000913/4110670‑4110938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑