Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,137,229	A→G	S143S (AGT→AGC)	yijF ←	DUF1287 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,137,229	0	A	G	100.0%	117.8 / NA	34	S143S (AGT→AGC)	yijF	DUF1287 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/16);  total (18/16)

TTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCAAAATTC  >  NC_000913/4137097‑4137361                                                                                                                                     |                                                                                                                                     ttcCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTgggg                                                                                                                                <  2:40120/139‑1 (MQ=255)  tcCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGt                                                                                                                               <  1:449331/139‑1 (MQ=255)   ctttttcgcCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGtt                                                                                                                              <  2:427479/133‑1 (MQ=255)                         aTGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTa                                                                                                        >  1:370321/1‑139 (MQ=255)                           gTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATc                                                                                                      >  1:770337/1‑139 (MQ=255)                               cACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATgg                                                                                                  >  2:523730/1‑139 (MQ=255)                               cACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATgg                                                                                                  >  2:166158/1‑139 (MQ=255)                                 ccAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATggcg                                                                                                <  1:550833/139‑1 (MQ=255)                                 ccAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTGATCATggcg                                                                                                >  1:474368/1‑139 (MQ=255)                                     cggcgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTa                                                                                            >  1:320900/1‑139 (MQ=255)                                        cgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAAc                                                                                         <  2:370321/139‑1 (MQ=255)                                        cgTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAAc                                                                                         >  1:799808/1‑139 (MQ=255)                                           aCCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAg                                                                                      <  2:320900/139‑1 (MQ=255)                                                               cTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCg                                                                  <  2:799808/139‑1 (MQ=255)                                                                    accaccCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGt                                                             >  1:246962/1‑139 (MQ=255)                                                                       accCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGAt                                                          <  2:317311/139‑1 (MQ=255)                                                                        ccccAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTGGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACGAGGTTACCAGATTAGGCACCCGACGCGGATc                                                         >  1:384580/1‑139 (MQ=255)                                                                                       gCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCgg                                          >  2:289051/1‑139 (MQ=255)                                                                                        cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCtg                                                                                                                       >  2:409754/1‑61 (MQ=255)                                                                                        cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCtg                                                                                                                       <  1:409754/61‑1 (MQ=255)                                                                                        cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCAt                                                                                                    <  2:42719/80‑1 (MQ=255)                                                                                        cccGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCAt                                                                                                    >  1:42719/1‑80 (MQ=255)                                                                                            ttATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCAc                                                                     <  1:192098/107‑1 (MQ=255)                                                                                            ttATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCAc                                                                     >  2:192098/1‑107 (MQ=255)                                                                                                      gCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                                >  1:724501/1‑54 (MQ=255)                                                                                                      gCCAGGAGACAATATCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGc                                                                                                                <  2:724501/54‑1 (MQ=255)                                                                                                                  tatCGCCCGCTTGATAGTCGCTGGGGTTCTTGCtggt                                                                                                                     >  2:226995/1‑37 (MQ=255)                                                                                                                  tatCGCCCGCTTGATAGTCGCTGGGGTTCTTGCtggt                                                                                                                     <  1:226995/37‑1 (MQ=255)                                                                                                                  tatCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAgg                                                                        <  2:212226/82‑1 (MQ=255)                                                                                                                  tatCGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAgg                                                                        >  1:212226/1‑82 (MQ=255)                                                                                                                     cGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAg                                                                         <  2:539888/78‑1 (MQ=255)                                                                                                                     cGCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAg                                                                         >  1:539888/1‑78 (MQ=255)                                                                                                                      gCCCGCTTGATAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCa           >  1:461757/1‑139 (MQ=255)                                                                                                                               aTAGTCGCTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCAAAAttc  <  1:289051/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     TTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTTGCTGGTGGGGCGTGTTTTATCATGGCGGCTAAACCAGGTTTCCAGATTAGGCACCCGACGGTGATCGATGTTGCTGTCCGGGCGCTTTAACTTCCATTTTTGCGGGTACTCAGCAAAATTC  >  NC_000913/4137097‑4137361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑