Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,279,634	G→A	V386I (GTT→ATT)	ghxP →	guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,279,634	0	G	A	100.0%	128.7 / NA	41	V386I (GTT→ATT)	ghxP	guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/17);  total (24/17)

GTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTG  >  NC_000913/4279507‑4279762                                                                                                                                |                                                                                                                                 gTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATg                                                                                                                       >  2:82303/1‑139 (MQ=255)  ttCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATgg                                                                                                                      <  2:622258/139‑1 (MQ=255)                 ttCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACgg                                                                                                       >  1:451226/1‑139 (MQ=255)                  tCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGc                                                                                                      >  2:736769/1‑139 (MQ=255)                      ctctcCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTa                                                                                                  >  2:741985/1‑139 (MQ=255)                         tcCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTc                                                                                               >  1:767259/1‑139 (MQ=255)                          ccGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAACTCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCa                                                                                              <  1:721841/139‑1 (MQ=255)                              tctctTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGt                                                                                          >  2:274462/1‑139 (MQ=255)                                  ttACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTg                                                                                      <  1:181620/139‑1 (MQ=255)                                        cGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTg                                                                                         <  2:739095/130‑1 (MQ=255)                                        cGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTg                                                                                         >  1:739095/1‑130 (MQ=255)                                          ttCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCt                                                                                       <  2:96205/130‑1 (MQ=255)                                          ttCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCt                                                                                       >  1:96205/1‑130 (MQ=255)                                          ttCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTaa                                                                              >  1:597659/1‑139 (MQ=255)                                          ttCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTaa                                                                              >  1:102866/1‑139 (MQ=255)                                          ttCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTaa                                                                              >  2:237110/1‑139 (MQ=255)                                            ccGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACa                                                                            <  2:597659/139‑1 (MQ=255)                                                  tATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTaa                                                                      <  2:102866/139‑1 (MQ=255)                                                         cGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGcat                                                               >  1:664861/1‑139 (MQ=255)                                                            cTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGt                                                                                                            <  1:394706/91‑1 (MQ=255)                                                            cTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGt                                                                                                            >  2:394706/1‑91 (MQ=255)                                                              ccGGCGCTGATGTACGTTCGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACACGCATCATGa                                                          <  2:540742/139‑1 (MQ=255)                                                                 gcgcTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCg                                                       >  2:42276/1‑139 (MQ=255)                                                                       aTGTACGTTGTGCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCTATGGTTGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATTATGATCGGCTTcg                                                 <  1:541479/139‑1 (MQ=255)                                                                            cGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTg                                                                                      <  2:26153/97‑1 (MQ=255)                                                                            cGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTg                                                                                      >  1:26153/1‑97 (MQ=255)                                                                                 gCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGTCGGGTCTGGTTAGGGTTGTATTCATCGTGCTGACCTGTCACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGt                                       >  2:234010/1‑139 (MQ=255)                                                                                 gCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGt                                       >  2:613496/1‑139 (MQ=255)                                                                                 gCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGAAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGt                                       >  2:228466/1‑139 (MQ=255)                                                                                               aGCAACGTGGCGAAAATCGATTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTgg                         >  1:472342/1‑139 (MQ=255)                                                                                               aGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTgg                         >  1:505803/1‑139 (MQ=255)                                                                                                    cGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGt                                                                                <  2:588112/79‑1 (MQ=255)                                                                                                    cGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGt                                                                                >  1:588112/1‑79 (MQ=255)                                                                                                    cGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTaa                                                                              >  1:60596/1‑81 (MQ=255)                                                                                                    cGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTaa                                                                              <  2:60596/81‑1 (MQ=255)                                                                                                    cGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTcc                    >  1:230036/1‑139 (MQ=255)                                                                                                                cGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGgcgc        <  2:767259/139‑1 (MQ=255)                                                                                                                cGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGgcgc        <  1:741985/139‑1 (MQ=255)                                                                                                                      tGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTg  <  2:505803/139‑1 (MQ=255)                                                                                                                            ttttATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGgcgc        <  2:56426/127‑1 (MQ=255)                                                                                                                            ttttATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGgcgc        >  1:56426/1‑127 (MQ=255)                                                                                                                                |                                                                                                                                 GTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTG  >  NC_000913/4279507‑4279762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑