Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,279,849 A→G intergenic (+21/‑131) ghxP → / → yjcE guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,279,8490AG100.0% 105.0 / NA 30intergenic (+21/‑131)ghxP/yjcEguanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/18);  total (12/18)

CGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGG  >  NC_000913/4279714‑4279983
                                                                                                                                       |                                                                                                                                      
cgcgACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCgg                                                                                                                                     <  2:552146/139‑1 (MQ=255)
               tGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGcc                                                                                                                      <  2:635995/139‑1 (MQ=255)
                                gCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCg                                                                                                     >  2:465462/1‑139 (MQ=255)
                                   aaTGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca                                                                                                 <  2:96621/139‑1 (MQ=255)
                                        cgcAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca                                                                                                 <  2:567230/135‑1 (MQ=255)
                                        cgcAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTAACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGcaca                                                                                                 >  1:567230/1‑135 (MQ=255)
                                                                 ttATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGaaaa                                                                    >  2:86742/1‑139 (MQ=255)
                                                                  tATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGaaaaa                                                                   >  1:407530/1‑139 (MQ=255)
                                                                     cGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAAt                                                                <  1:465462/139‑1 (MQ=255)
                                                                        cGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCg                                                                                                                                      <  1:84454/66‑1 (MQ=255)
                                                                          tGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGaa                                                           >  2:282474/1‑139 (MQ=255)
                                                                            gcgcTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGc                                                                                                                       <  1:566991/77‑1 (MQ=255)
                                                                            gcgcTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGc                                                                                                                       >  2:566991/1‑77 (MQ=255)
                                                                                    gACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATa                                                 <  1:314292/139‑1 (MQ=255)
                                                                                    gACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATa                                                 <  2:669785/139‑1 (MQ=255)
                                                                                                     gCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATa                                                 >  1:642687/1‑122 (MQ=255)
                                                                                                     gCTGGGCTATCTAATCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATa                                                 <  2:642687/122‑1 (MQ=255)
                                                                                                       tGGGCTATCTAATCTTTCATCGGATTATGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTGTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCCTa                              <  1:596515/139‑1 (MQ=255)
                                                                                                             aTCTAATCTTTCACCGGATTCTGAAAGCGGGAGGCTATGGCTGCCCGTTTTTAATTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAAGGAGAATGCAGGCATAACGTCTTTCTCAGATCATAAAAAAc                        <  1:414983/139‑1 (MQ=255)
                                                                                                                   tCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGcc                                                                                                                      <  1:608683/39‑1 (MQ=255)
                                                                                                                   tCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGcc                                                                                                                      >  2:608683/1‑39 (MQ=255)
                                                                                                                   tCTTTCATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCa                  <  2:441618/139‑1 (MQ=255)
                                                                                                                        cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATa                                                 >  2:299469/1‑103 (MQ=255)
                                                                                                                        cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATa                                                 <  1:299469/103‑1 (MQ=255)
                                                                                                                        cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCa                                     >  2:336499/1‑115 (MQ=255)
                                                                                                                        cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCa                                     <  1:336499/115‑1 (MQ=255)
                                                                                                                        cATCGGATTCTGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAg             >  1:434198/1‑139 (MQ=255)
                                                                                                                                  tGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCtt                                                                                     >  2:323294/1‑57 (MQ=255)
                                                                                                                                  tGAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCtt                                                                                     <  1:323294/57‑1 (MQ=255)
                                                                                                                                   gAAAGCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATgg  <  2:407530/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGG  >  NC_000913/4279714‑4279983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: